INTEGRATION AND EXPRESSION OF A RABBIT LIVER CYTOCHROME-P-450 GENE IN TRANSGENIC NICOTIANA-TABACUM

被引：40

作者：

SAITO, K ^{[1
]}

NOJI, M ^{[1
]}

OHMORI, S ^{[1
]}

IMAI, Y ^{[1
]}

MURAKOSHI, I ^{[1
]}

机构：

[1] UNIV OSAKA PREFECTURE,FAC AGR,SAKAI,OSAKA 591,JAPAN

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 16期

关键词：

SENESCENCE; METABOLISM OF NICOTINE; AGROBACTERIUM-TUMEFACIENS; TI-PLASMID VECTOR; PROMOTER FOR MANNOPINE SYNTHASE;

D O I：

10.1073/pnas.88.16.7041

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cytochrome P-450 is involved in the oxidative metabolism of a broad range of substrates. We have made a chimeric construct, pSN002, containing the cDNA for rabbit liver cytochrome P-450 (IIC14) under the control of the TR2' promoter for mannopine synthase in the Agrobacterium Ti plasmid. Nicotiana tabacum was transformed with Agrobacterium tumefaciens harboring a cointegrated plasmid pSN002::pGV2260. The presence of mRNA and of the translated protein from the chimeric cytochrome P-450 gene in transgenic plants was confirmed by RNA blot hybridization and by Western blot and immunohistochemical analyses, respectively. The transformants in which the foreign cytochrome P-450 protein is expressed show marked phenotypic changes, notably a tendency rapidly to senesce. We detected 2-propenylpyrrolidine, a degradative metabolite of nicotine alkaloids, in transgenic tobacco showing this pronounced phenotypic change. Such metabolism is likely to be due to the effect of senescence and not directly to the presence of the cytochrome P-450.

引用

页码：7041 / 7045

页数：5

共 43 条

[11] THE PROBLEM OF CHEMICALLY REACTIVE METABOLITES [J].