CONTRIBUTIONS OF MULTIPLE CALCIUM-CHANNEL TYPES TO GABAERGIC TRANSMISSION IN RAT CULTURED HIPPOCAMPAL-NEURONS

被引：23

作者：

OHNOSHOSAKU, T ^{[1
]}

HIRATA, K ^{[1
]}

SAWADA, S ^{[1
]}

YAMAMOTO, C ^{[1
]}

机构：

[1] TOYAMA CHEM CO LTD,RES LABS,TOYAMA 930,JAPAN

来源：

NEUROSCIENCE LETTERS | 1994年 / 181卷 / 1-2期

关键词：

HIPPOCAMPAL NEURON; GABAERGIC TRANSMISSION; TRANSMITTER RELEASE; INHIBITORY SYNAPSE; CA2+ CHANNEL; CA2+ CHANNEL BLOCKER;

D O I：

10.1016/0304-3940(94)90580-0

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Effects of Ca2+ channel blockers on GABAergic transmission were examined with rat cultured hippocampal neurons. The GABAergic synaptic currents (IPSCs) evoked by electrical stimulation of single presynaptic neurons were recorded from voltage-clamped postsynaptic neurons. The amplitude of IPSCs was not changed by 10 mu M nifedipine (L-type Ca2+ channel blocker), but was decreased irreversibly to 37% of control by 1 mu M omega-conotoxin GVIA (N-type blocker, CgTX), to 67% by 0.1 mu M omega-agatoxin IVA (P-type blocker, AGTX), and to 1% by both toxins. From these data and the Ca2+-dependence of IPSCs, the contributions of CgTX-sensitive and AGTX-sensitive Ca2+ channels to the transmission were estimated at 59% and 36%, respectively.

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页码：145 / 148

页数：4

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