PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE (PACAP) AND VASOACTIVE-INTESTINAL-PEPTIDE STIMULATE 2 SIGNALING PATHWAYS IN CHO CELLS STABLY TRANSFECTED WITH THE SELECTIVE TYPE-I PACAP RECEPTOR

被引：35

作者：

DELPORTE, C ^{[1
]}

POLOCZEK, P ^{[1
]}

DENEEF, P ^{[1
]}

VERTONGEN, P ^{[1
]}

CICCARELLI, E ^{[1
]}

SVOBODA, M ^{[1
]}

HERCHUELZ, A ^{[1
]}

WINAND, J ^{[1
]}

ROBBERECHT, P ^{[1
]}

机构：

[1] FREE UNIV BRUSSELS,SCH MED,PHARMACOL LAB,BRUSSELS,BELGIUM

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1995年 / 107卷 / 01期

关键词：

PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE (PACAP) RECEPTOR CYTOSOLIC FREE CALCIUM; INOSITOL PHOSPHATE; CYCLIC AMP; CHINESE HAMSTER OVARY CELLS;

D O I：

10.1016/0303-7207(94)03424-R

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The properties of the pituitary adenylate cyclase activating polypeptide (PACAP) type I receptor were studied on a clone of Chinese hamster ovary cells (CHO) stably transfected with the recombinant receptor. PACAP(1-27), PACAP(1-38) and VIP inhibited [I-125-acetyi-His(1)]PACAP(1-27) binding, stimulated cyclic AMP and inositol phosphates production and induced [Ca2+](i) increase with the same order of potency: PACAP(1-27)=PACAP(1-38) > VIP. The concentrations required for half maximal receptor occupancy, IP3- and [Ca2+](i) increase were not different for both PACAPs (1 nM) and 100-fold higher than those required for cyclic AMP increase (0.010 nM). These data suggest that the occupancy of a portion of the total receptors available was sufficient for maximal cyclic AMP production but not for maximal IP3 production. It is concluded that the possibility of the type I PACAP receptor being coupled to a transduction pathway is not located at the level of the ligand but rather at the level of the G-proteins.

引用

页码：71 / 76

页数：6

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