EVALUATION OF SILANOL-DEACTIVATED SILICA-BASED REVERSED PHASES FOR LIQUID-CHROMATOGRAPHY OF ERYTHROMYCIN

被引：34

作者：

PAESEN, J ^{[1
]}

CLAEYS, P ^{[1
]}

ROETS, E ^{[1
]}

HOOGMARTENS, J ^{[1
]}

机构：

[1] CATHOLIC UNIV LEUVEN,INST FARMACEUT WETENSCHAPPEN,FARMACEUT CHEM LAB,VAN EVENSTR 4,B-3000 LOUVAIN,BELGIUM

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 630卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(93)80446-F

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The suitability of eleven silanol-deactivated reversed phases for the liquid chromatography of erythromycin was investigated. The selectivity and efficiency of each stationary phase were examined. The performance was compared to that of a non-deactivated C18 silica-based reversed-phase material, Hypersil C18 (5 mum). Two types of mobile phases were used, one containing no tetrabutylammonium (TBA) and the other containing TBA. Addition of TBA as a silanol-blocking agent improved the theoretical plate number and symmetry factor of the peaks corresponding to erythromycin A (EA) and erythromycin A enol ether for all the deactivated reversed phases. These results are an indication of the presence of some residual silanol activity in these phases. Separation of erythromycin E and EA was achieved on only two of the eleven phases. The selectivity was always poorer than that obtained in a previously described method using a poly(styrene-divinylbenzene) stationary phase.

引用

页码：117 / 122

页数：6

共 11 条

[1] CHEMICALLY BONDED PHASES FOR THE REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF BASIC SUBSTANCES [J].