INCORPORATION OF (R) AND (S)-3',4'-SECO-THYMIDINE INTO OLIGODEOXYNUCLEOTIDES - HYBRIDIZATION PROPERTIES AND ENZYMATIC STABILITY

被引：41

作者：

NIELSEN, P

KIRPEKAR, F

WENGEL, J

机构：

[1] ODENSE UNIV,DEPT CHEM,DK-5230 ODENSE,DENMARK

[2] ODENSE UNIV,DEPT MOLEC BIOL,DK-5230 ODENSE,DENMARK

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 05期

关键词：

D O I：

10.1093/nar/22.5.703

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Novel flexible oligodeoxynucleotide analogues containing (R)- and (S)-3',4'-seco-thymidine were synthesized on an automated DNA-synthesizer using the phosphoramidite approach. Oligodeoxynucleotide analogues (17-mers) having one or three modifications in the middle or one or two modifications in the ends were evaluated with respect to hybridization properties and enzymatic stability. 3'-End-modified oligomers were stable towards 3'-exonuclease degradation and displayed acceptable hybridization properties.

引用

页码：703 / 710

页数：8

共 37 条

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