Structures of decasaccharide and tridecasaccharide tetraphosphates isolated by strong alkaline degradation of O-deacylated lipopolysaccharide of Pseudomonas fluorescens strain ATCC 49271

被引：25

作者：

Knirel, YA

Grosskurth, H

Helbig, JH

Zahringer, U

机构：

[1] FORSCHUNGSINST BORSTEL,INST EXPTL BIOL & MED,D-23845 BORSTEL,GERMANY

[2] RUSSIAN ACAD SCI,ND ZELINSKII ORGAN CHEM INST,MOSCOW 117913,RUSSIA

[3] MAX PLANCK INST MED RES,D-69120 HEIDELBERG,GERMANY

[4] TU KLINIKUM DRESDEN,INST MED MIKROBIOL & HYG,D-01307 DRESDEN,GERMANY

来源：

CARBOHYDRATE RESEARCH | 1995年 / 279卷

关键词：

lipopolysaccharide; core oligosaccharide; Pseudomonas; structure; degradation; NMR spectroscopy;

D O I：

10.1016/0008-6215(95)00274-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mild hydrazinolysis of Pseudomonas fluorescens strain ATCC 49271 lipopolysaccharide (LPS) followed by strong alkaline degradation and purification by anion-exchange HPLC resulted in two phosphorylated oligosaccharides (1 and 2). On the basis of compositional analysis and H-1, C-13, and P-31 NMR spectroscopy, including 2D correlation spectroscopy (COSY), 2D rotating frame NOE spectroscopy (ROESY), and 2D inverse mode H-detected heteronuclear H-1-C-13 and H-1-(31)p correlation spectroscopy, the following two structures (1 and 2) could be identified

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页码：215 / 226

页数：12

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