ACTIVATION OF ASPARTASE BY SITE-DIRECTED MUTAGENESIS

被引：21

作者：

MURASE, S ^{[1
]}

TAKAGI, JS ^{[1
]}

HIGASHI, Y ^{[1
]}

IMAISHI, H ^{[1
]}

YUMOTO, N ^{[1
]}

TOKUSHIGE, M ^{[1
]}

机构：

[1] KYOTO UNIV,FAC SCI,DEPT CHEM,KYOTO 606,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 177卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)91999-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To elucidate the role of sulfhydryl groups in the enzymatic reaction of the aspartase from Escherichia coli, we used site-directed mutagenesis which showed that the enzyme was activated by replacement of Cys-430 with a tryptophan. This mutation produced functional alterations without appreciable structural change: The kcat values became 3-fold at pH 6.0; the Hill coefficient values became higher under both pH conditions; the dependence of enzyme activity on divalent metal ions increased; and hydroxylamine, a good substrate for the wild-type enzyme, proved a poor substrate for the mutant. © 1991.

引用

页码：414 / 419

页数：6

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