RETENTION CHARACTERISTICS OF A BETA-CYCLODEXTRIN POLYMER-COATED LIQUID-CHROMATOGRAPHIC COLUMN

A silica based, beta-cyclodextrin polymer-coated liquid chromatographic column was prepared and its retention characteristics were determined using 28 ring-substituted phenol derivatives as solutes and methanol-water mixtures as eluents. The column showed a good separation power; the log k' value decreased linearly with increasing methanol concentration. Stepwise regression analysis demonstrated that the lipophilicity does not determine the retention on the beta-cyclodextrin polymer. Accordingly, the retention mechanism deviates from that of traditional reversed-phase columns. The steric characteristics and to a lesser extent the electronic parameters of the solutes have the greatest impact on the retention, which means that the inclusion complex formation also prevails after polymerization, resulting in a modified retention behaviour of the complexed solutes.