MOLECULAR CHARACTERIZATION OF TOBACCO CDNAS ENCODING 2 SMALL GTP-BINDING PROTEINS

被引:46
作者
DALLMANN, G
STICHER, L
MARSHALLSAY, C
NAGY, F
机构
[1] FRIEDRICH MIESCHER INST,POB 2543,CH-4002 BASEL,SWITZERLAND
[2] HUNGARIAN ACAD SCI,BIOL RES CTR,H-6701 SZEGED,HUNGARY
关键词
GTP-BINDING PROTEINS; TOBACCO; RAS-RELATED GENES; TISSUE SPECIFICITY;
D O I
10.1007/BF00027080
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have isolated two cDNAs encoding small GTP-binding proteins from leaf cDNA libraries. These cDNAs encode distinct proteins which show considerable homology to members of the ras superfamily. Np-ypt3, a 1044 bp long Nicotiana plumbaginifolia cDNA, encodes a 24.4 kDa protein which shows 65 % amino acid sequence similarity to the Schizosaccharomyces pombe ypt3 protein. The N-ypt3 gene is differentially expressed in mature flowering plants. Expression of this gene is weak in leaves, higher in stems and roots, but highest in petals, stigmas and stamens. Nt-rab5, a 712 bp long Nicotiana tabacum SR1 cDNA, encodes a 21.9 kDa protein which displays 65% amino acid sequence similarity to mammalian rab5 proteins. The expression pattern of the Nt-rab5 gene is very similar to that of the Np-ypt3 gene. The Nt-rab5 gene is virtually not expressed in leaves, higher in stems and roots, and highest in flowers. Both the Nt-rab5 and Np-ypt3 proteins were expressed in Escherichia coli and shown to bind GTP.
引用
收藏
页码:847 / 857
页数:11
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