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KU80 - PRODUCT OF THE XRCC5 GENE AND ITS ROLE IN DNA-REPAIR AND V(D)J RECOMBINATION

被引：581
作者：
TACCIOLI, GE
GOTTLIEB, TM
BLUNT, T
PRIESTLEY, A
DEMENGEOT, J
MIZUTA, R
LEHMANN, AR
ALT, FW
JACKSON, SP
JEGGO, PA
机构：
[1] UNIV SUSSEX,MRC,CELL MUTAT UNIT,BRIGHTON BN1 9RR,ENGLAND
[2] CHILDRENS HOSP,HOWARD HUGHES MED INST,BOSTON,MA 02115
[3] HARVARD UNIV,SCH MED,DEPT GENET,BOSTON,MA
[4] UNIV CAMBRIDGE,WELLCOME CRC INST,CAMBRIDGE CB2 1QR,ENGLAND
[5] UNIV CAMBRIDGE,DEPT ZOOL,CAMBRIDGE CB2 1QR,ENGLAND
来源：
SCIENCE | 1994年 / 265卷 / 5177期
基金：
英国惠康基金;
关键词：
D O I：
10.1126/science.8073286
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The radiosensitive mutant xrs-6, derived from Chinese hamster ovary cells, is defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. The human XRCC5 DNA repair gene, which complements this mutant, is shown here through genetic and biochemical evidence to be the 80-kilodalton subunit of the Ku protein. Ku binds to free double-stranded DNA ends and is the DNA-binding component of the DNA-dependent protein kinase. Thus, the Ku protein is involved in DNA repair and in V(D)J recombination, and these results may also indicate a role for the Ku-DNA-dependent protein kinase complex in those same processes.
引用
收藏
页码：1442 / 1445
页数：4
相关论文
共 48 条
[31]   JUNCTIONAL SEQUENCES OF T-CELL RECEPTOR GAMMA-DELTA-GENES - IMPLICATIONS FOR GAMMA-DELTA-T-CELL LINEAGES AND FOR A NOVEL INTERMEDIATE OF V-(D)-J JOINING [J].
LAFAILLE, JJ ;
DECLOUX, A ;
BONNEVILLE, M ;
TAKAGAKI, Y ;
TONEGAWA, S .
CELL, 1989, 59 (05) :859-870
[32]   THE DEFECT IN MURINE SEVERE COMBINED IMMUNE-DEFICIENCY - JOINING OF SIGNAL SEQUENCES BUT NOT CODING SEGMENTS IN V(D)J RECOMBINATION [J].
LIEBER, MR ;
HESSE, JE ;
LEWIS, S ;
BOSMA, GC ;
ROSENBERG, N ;
MIZUUCHI, K ;
BOSMA, MJ ;
GELLERT, M .
CELL, 1988, 55 (01) :7-16
[33]   THE SCID DEFECT AFFECTS THE FINAL STEP OF THE IMMUNOGLOBULIN VDJ RECOMBINASE MECHANISM [J].
MALYNN, BA ;
BLACKWELL, TK ;
FULOP, GM ;
RATHBUN, GA ;
FURLEY, AJW ;
FERRIER, P ;
HEINKE, LB ;
PHILLIPS, RA ;
YANCOPOULOS, GD ;
ALT, FW .
CELL, 1988, 54 (04) :453-460
[34]  
MIMORI T, 1986, J BIOL CHEM, V261, P2274
[35]   RAG-1 AND RAG-2, ADJACENT GENES THAT SYNERGISTICALLY ACTIVATE V(D)J RECOMBINATION [J].
OETTINGER, MA ;
SCHATZ, DG ;
GORKA, C ;
BALTIMORE, D .
SCIENCE, 1990, 248 (4962) :1517-1523
[36]   V(D)J RECOMBINATION IN MAMMALIAN-CELL MUTANTS DEFECTIVE IN DNA DOUBLE-STRAND BREAK REPAIR [J].
PERGOLA, F ;
ZDZIENICKA, MZ ;
LIEBER, MR .
MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (06) :3464-3471
[37]   SPECIFIC TRANSCRIPTION FROM THE ADENOVIRUS E2E PROMOTER BY RNA POLYMERASE-III REQUIRES A SUBPOPULATION OF TFIID [J].
PRUZAN, R ;
CHATTERJEE, PK ;
FLINT, SJ .
NUCLEIC ACIDS RESEARCH, 1992, 20 (21) :5705-5712
[38]   INVOLVEMENT OF THE KU AUTOANTIGEN IN THE CELLULAR-RESPONSE TO DNA DOUBLE-STRAND BREAKS [J].
RATHMELL, WK ;
CHU, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (16) :7623-7627
[39]   A DNA END-BINDING FACTOR INVOLVED IN DOUBLE-STRAND BREAK REPAIR AND V(D)J RECOMBINATION [J].
RATHMELL, WK ;
CHU, G .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (07) :4741-4748
[40]   A FAMILY OF OCTAMER-SPECIFIC PROTEINS PRESENT DURING MOUSE EMBRYOGENESIS - EVIDENCE FOR GERMLINE-SPECIFIC EXPRESSION OF AN OCT FACTOR [J].
SCHOLER, HR ;
HATZOPOULOS, AK ;
BALLING, R ;
SUZUKI, N ;
GRUSS, P .
EMBO JOURNAL, 1989, 8 (09) :2543-2550
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