MOLECULAR-WEIGHT AND STRUCTURAL STUDIES ON CEPHALOPOD RHODOPSIN

被引:31
作者
NASHIMA, K [1 ]
MITSUDO, M [1 ]
KITO, Y [1 ]
机构
[1] OSAKA KYOIKU UNIV, DEPT HLTH SCI, OSAKA 547, JAPAN
关键词
(Squid); Rhodopsin structure; Visual pigment;
D O I
10.1016/0005-2795(79)90095-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The protein moiety of squid (Watasenia scintillans) rhodopsin has been shown to have a molecular weight of 46 800 by means of amino acid analysis. This value was comparable to the value (51 000) obtained from SDS-polyacrylamide gel electrophoresis. After the squid eyes were incubated at 10°C for 8 days, the rhodopsin showed a molecular weight of 39 000 on electrophoresis. The smaller molecular weight was ascertained by amino acid analysis of the rhodopsin; and may result from autolysis by the lysosomal enzyme. The rhodopsin in rhabdomeric membranes and in detergent solution was treated with chymotrypsin, papain or subtilisin. These enzymes first produced the 39 000 dalton rhodopsin and then cleaved this into the 25 000 and 14 000 dalton peptides without bleaching. The rhodopsin was attacked by proteases and readily lost an approx. 12 000 dalton peptide portion. This portion included the COOH-terminal and was rich in glutamic acid, proline, glycine, alanine and tyrosine residues. © 1979.
引用
收藏
页码:155 / 168
页数:14
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