PHAGOCYTOSIS ASSOCIATED CHEMILUMINESCENCE OF HEMOCYTES IN MYTILUS-EDULIS (BIVALVIA)

被引：73

作者：

NOEL, D

BACHERE, E

MIALHE, E

机构：

[1] IFREMER, CNRS/UM2, Défense et Résistance chez les Invertébrés Marins, F-34095 Montpellier Cédex 5

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 1993年 / 17卷 / 06期

关键词：

MOLLUSK; MYTILUS; HEMOCYTES; PHAGOCYTOSIS; RESPIRATORY BURST; CHEMILUMINESCENCE; OXYGEN RADICALS; MONOCLONAL ANTIBODY; ISOPYCNIC CENTRIFUGATION; SUBPOPULATION;

D O I：

10.1016/S0145-305X(05)80004-7

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The respiratory burst associated with phagocytosis by Mytilus edulis hemocytes was investigated by measurement of luminol-dependent chemiluminescence (LDCL). After experimental parameters (number of cells, quantity of stimulus) were determined, the biochemical mechanisms involved in the chemiluminescent process were investigated using inhibitors of oxygen radicals and enzymes. In particular, catechol-like phenols suggested the involvement of NADPH-oxidase and peroxidase in oxidative metabolism of mussel hemocytes. The variability of LDCL response observed among individuals and separated hemocyte subpopulations strongly suggests a variable immunocapacity depending on hemogram composition. Using a specific monoclonal antibody to discriminate different hemocyte types, the eosinophilic granulocytes appeared to exhibit the highest LDCL activity.

引用

页码：483 / 493

页数：11

共 43 条

[11] ASPECTS OF SUBSTRATE UTILIZATION AND ENERGY REQUIREMENT DURING MOLLUSCAN PHAGOCYTOSIS
CHENG, TC
[J]. JOURNAL OF INVERTEBRATE PATHOLOGY, 1976, 27 (02) : 263 - 268
[12] ANTIOXIDANT PROPERTIES OF NATURAL HYDROQUINONES FROM THE MARINE COLONIAL TUNICATE APLIDIUM-CALIFORNICUM
COTELLE, N
MOREAU, S
BERNIER, JL
CATTEAU, JP
HENICHART, JP
[J]. FREE RADICAL BIOLOGY AND MEDICINE, 1991, 11 (01) : 63 - 68
[13] DECHATELET LR, 1982, J IMMUNOL, V129, P1589
[14] THE PRODUCTION OF TOXIC OXYGEN METABOLITES BY HEMOCYTES OF DIFFERENT SNAIL SPECIES
DIKKEBOOM, R
VANDERKNAAP, WPW
VANDENBOVENKAMP, W
TIJNAGEL, JMGH
BAYNE, CJ
[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 1988, 12 (03) : 509 - 520
[15] HEMOCYTES OF THE POND SNAIL LYMNAEA-STAGNALIS GENERATE REACTIVE FORMS OF OXYGEN
DIKKEBOOM, R
TIJNAGEL, JMGH
MULDER, EC
VANDERKNAAP, WPW
[J]. JOURNAL OF INVERTEBRATE PATHOLOGY, 1987, 49 (03) : 321 - 331
[16] NEGATIVE SELECTION OF HUMAN MONOCYTES USING MAGNETIC PARTICLES COVERED BY ANTILYMPHOCYTE ANTIBODIES
FLO, RW
NAESS, A
LUNDJOHANSEN, F
MAEHLE, BO
SJURSEN, H
LEHMANN, V
SOLBERG, CO
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1991, 137 (01) : 89 - 94
[17] GENNARO R, 1991, BLOOD CELL BIOCH, V3, P335
[18] GOMEZ CL, 1992, 5TH INT C PATH MAR A
[19] Hervio D., 1989, Developmental and Comparative Immunology, V13, P449, DOI 10.1016/0145-305X(89)90176-6
[20] Klebanoff S. J., 1978, NEUTROPHIL FUNCTION, P810

← 1 2 3 4 5 →