A PLANT METALLOTHIONEIN PRODUCED IN ESCHERICHIA-COLI

被引：82

作者：

KILLE, P

WINGE, DR

HARWOOD, JL

KAY, J

机构：

[1] UNIV WALES COLL CARDIFF,DEPT BIOCHEM,POB 903,CARDIFF CF1 1ST,WALES

[2] UNIV UTAH,SCH MED,DIV HAEMATOL ONCOL,SALT LAKE CITY,UT 84132

来源：

FEBS LETTERS | 1991年 / 295卷 / 1-3期

关键词：

PEA METALLOTHIONEIN; CLONING; PCR; EXPRESSION IN ESCHERICHIA-COLI; RECOMBINANT METALLOTHIONEIN; EXTENDED LINKER REGION; PROTEOLYTIC NICKING; CYSTEINE-RICH REGION; METAL-BINDING;

D O I：

10.1016/0014-5793(91)81411-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A metallothionein cDNA was generated from pea (Pisum sativum L.) roots, amplified by PCR and inserted into a plasmid for expression in E. coli. Purification of the resultant product generated 3 pools of cadmium-containing material after DEAE-cellulose chromatography. The amino acid composition of each was in excellent agreement with that predicted for pea metallothionein. A cadmium content of approximately 6 g.atoms per mole of protein was estimated. N-terminal sequence analysis revealed that the recombinant molecule had been proteolysed within the extended region linking the 2 cysteine-rich (putative) metal-binding regions. The significance of these findings in terms of the protein folding/targeting of the molecule are considered.

引用

页码：171 / 175

页数：5

共 10 条

[1] CORRELATION BETWEEN THE DNA SUPERCOILING AND THE INITIATION OF TRANSCRIPTION BY ESCHERICHIA-COLI RNA-POLYMERASE INVITRO - ROLE OF THE SEQUENCES UPSTREAM OF THE PROMOTER REGION [J].