SUBSTRATE RECOGNITION DOMAIN OF THE GAL2 GALACTOSE TRANSPORTER IN YEAST SACCHAROMYCES-CEREVISIAE AS REVEALED BY CHIMERIC GALACTOSE-GLUCOSE TRANSPORTERS

被引：52

作者：

NISHIZAWA, K ^{[1
]}

SHIMODA, E ^{[1
]}

KASAHARA, M ^{[1
]}

机构：

[1] TEIKYO UNIV, SCH MED, BIOPHYS LAB, HACHIOJI, TOKYO 19203, JAPAN

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1995年 / 270卷 / 06期

关键词：

D O I：

10.1074/jbc.270.6.2423

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Gal2 galactose transporter takes up galactose in yeast. A homologous glucose transporter from the same organism, Hxt2, was selected, and various chimeras between these two transporters were constructed by making use of homologous recombination in Escherichia coli. Comparison of the galactose transport activities of three series of chimeras enabled us to positively identify a crucial substrate recognition region of 101 amino acids that lies close to the carboxyl terminus of the Gal2 transporter.

引用

页码：2423 / 2426

页数：4

共 38 条

[21] MUTATIONAL ANALYSIS OF THE SNF3 GLUCOSE TRANSPORTER OF SACCHAROMYCES-CEREVISIAE [J].

MARSHALLCARLSON, L ;

CELENZA, JL ;

LAURENT, BC ;

CARLSON, M .

MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (03) :1105-1115

[22]

MORI H, 1994, J BIOL CHEM, V269, P11578

[23] FACILITATIVE GLUCOSE TRANSPORTERS [J].

MUECKLER, M .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 219 (03) :713-725

[24]

MUECKLER M, 1994, J BIOL CHEM, V269, P17765

[25]

MUECKLER M, 1994, J BIOL CHEM, V269, P20533

[26] YEAST GALACTOSE PERMEASE IS RELATED TO YEAST AND MAMMALIAN GLUCOSE TRANSPORTERS [J].

NEHLIN, JO ;

CARLBERG, M ;

RONNE, H .

GENE, 1989, 85 (02) :313-319

[27] FUNCTIONAL STRUCTURES OF THE RECA PROTEIN FOUND BY CHIMERA ANALYSIS [J].

OGAWA, T ;

SHINOHARA, A ;

OGAWA, H ;

TOMIZAWA, J .

JOURNAL OF MOLECULAR BIOLOGY, 1992, 226 (03) :651-660

[28] C-TERMINAL TRUNCATED GLUCOSE TRANSPORTER IS LOCKED INTO AN INWARD-FACING FORM WITHOUT TRANSPORT ACTIVITY [J].

OKA, Y ;

ASANO, T ;

SHIBASAKI, Y ;

LIN, JL ;

TSUKUDA, K ;

KATAGIRI, H ;

AKANUMA, Y ;

TAKAKU, F .

NATURE, 1990, 345 (6275) :550-553

[29] CHARACTERISTICS OF GALACTOSE TRANSPORT IN SACCHAROMYCES-CEREVISIAE CELLS AND RECONSTITUTED LIPID VESICLES [J].

RAMOS, J ;

SZKUTNICKA, K ;

CIRILLO, VP .

JOURNAL OF BACTERIOLOGY, 1989, 171 (06) :3539-3544

[30]

ROSE MD, 1991, METHOD ENZYMOL, V194, P195

← 1 2 3 4 →