USE OF 2,6-DINITRO-4-TRIFLUOROMETHYLBENZENESULFONIC ACID AS A NOVEL DERIVATIZING REAGENT FOR THE ANALYSIS OF CATECHOLAMINES, HISTAMINES AND RELATED AMINES BY GAS-CHROMATOGRAPHY WITH ELECTRON-CAPTURE DETECTION

被引:28
作者
DOSHI, PS [1 ]
EDWARDS, DJ [1 ]
机构
[1] UNIV PITTSBURGH, WESTERN PSYCHIAT INST & CLIN, DEPT PSYCHIAT, PITTSBURGH, PA 15261 USA
来源
JOURNAL OF CHROMATOGRAPHY | 1979年 / 176卷 / 03期
关键词
D O I
10.1016/S0021-9673(00)89453-4
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
We have found that 2,6-dinitro-4-trifluoromethylbenzensulfonic acid reacts rapidly and specifically with primary amines at room temperature. We have used this reagent for derivatizing phenylethylamines, including catecholamines, and histamine and 1-methylhistamine. After the reaction, hydroxyl groups were derivatized to form the corresponding trimethylsilyl ethers, and the final derivatives were analyzed by gas chromatography with electron-capture detection. These derivatives are stable, possess excellent gas chromatographic properties and are detected with high sensitivity. We have applied this method to the analysis of histamine and 1-methylhistamine in human urine. © 1979.
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页码:359 / 366
页数:8
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