DIRECT ENANTIOMERIC SEPARATION OF THE 4 STEREOISOMERS OF NADOLOL USING NORMAL-PHASE AND REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH CHIRALPAK-AD

Two enantiomeric separations have been developed which resolve the four stereoisomers of nadolol on Chiralpak AD using high-performance liquid chromatography. The normal-phase method uses hexane, ethanol and diethylamine to baseline resolve all four stereoisomers. The reversed-phase method uses ethanol, water and diethylamine. Both methods were optimized by investigating different mobile phase modifiers, flow-rates and column temperatures. Accuracy and reproducibility were determined for quantitating levels of racemate A and racemate B using the normal-phase method.