ASSAY FOR PLASMA-MEMBRANE PHOSPHATASE-ACTIVITY IN POPULATIONS OF INDIVIDUAL CELLS

被引：10

作者：

WATSON, JV ^{[1
]}

WORKMAN, P ^{[1
]}

CHAMBERS, SH ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,SCH MED,MRC,DEPT RADIOTHERAPEUT,CAMBRIDGE CB2 2QH,ENGLAND

来源：

BIOCHEMICAL PHARMACOLOGY | 1979年 / 28卷 / 06期

关键词：

D O I：

10.1016/0006-2952(79)90364-2

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Flow cytofluorimetric techniques have been used to assay plasma membrane phosphatase activity in populations of individual mouse mammary tumour cells. A new mathematical treatment, involving 'steady-state' kinetic assumptions to analyse the asymptotic portions of the progress curves, gave results which were compatible with normal Michaelian kinetics for the hydrolysis of the fluorogenic substrate 3-O-methyl fluorescein phosphate. The potential application of the methodology in the field of latent anti-neoplastic drugs is discussed. © 1979.

引用

页码：821 / 827

页数：7

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