ASSAY FOR PLASMA-MEMBRANE PHOSPHATASE-ACTIVITY IN POPULATIONS OF INDIVIDUAL CELLS

被引：10

作者：

WATSON, JV ^{[1
]}

WORKMAN, P ^{[1
]}

CHAMBERS, SH ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,SCH MED,MRC,DEPT RADIOTHERAPEUT,CAMBRIDGE CB2 2QH,ENGLAND

来源：

BIOCHEMICAL PHARMACOLOGY | 1979年 / 28卷 / 06期

关键词：

D O I：

10.1016/0006-2952(79)90364-2

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Flow cytofluorimetric techniques have been used to assay plasma membrane phosphatase activity in populations of individual mouse mammary tumour cells. A new mathematical treatment, involving 'steady-state' kinetic assumptions to analyse the asymptotic portions of the progress curves, gave results which were compatible with normal Michaelian kinetics for the hydrolysis of the fluorogenic substrate 3-O-methyl fluorescein phosphate. The potential application of the methodology in the field of latent anti-neoplastic drugs is discussed. © 1979.

引用

页码：821 / 827

页数：7

共 25 条

[11]

MORTON RK, 1965, COMPREHENSIVE BIOCH, V16, pCH2

[12]

ROCKWELL SC, 1972, J NATL CANCER I, V49, P735

[13]

ROSS WCJ, 1974, ANTINEOPLASTIC IMM 1, V38, pCH3

[14]

ROTTMAN B, 1966, P NATN ACAD SCI US, V55, P134

[15]

SELIGMAN AM, 1975, CANCER CHEMOTH REP 1, V59, P233

[16] CHANGES IN CELL-PROLIFERATION KINETICS OCCURRING DURING LIFE-HISTORY OF MONOLAYER-CULTURES OF A MOUSE TUMOR-CELL LINE [J].

TWENTYMAN, PR ;

WATSON, JV ;

BLEEHEN, NM ;

ROWLES, PM .

CELL AND TISSUE KINETICS, 1975, 8 (01) :41-50

[17] DIFFERENT ESTERASE-ACTIVITIES OF EXPONENTIAL AND PLATEAU PHASES OF EMT6 CELLS MONITORED BY FLOW CYTOFLUORIMETRY [J].

WATSON, JV ;

WORKMAN, P ;

CHAMBERS, SH .

BRITISH JOURNAL OF CANCER, 1978, 37 (03) :397-402

[18]

WATSON JV, 1977, FEBS LETT, V81, P179

[19] APPLICATION OF AGE DISTRIBUTION THEORY IN ANALYSIS OF CYTOFLUORIMETRIC DNA HISTOGRAM DATA [J].

WATSON, JV .

CELL AND TISSUE KINETICS, 1977, 10 (02) :157-169

[20] CELL-CYCLE ANALYSIS INVITRO USING FLOW CYTO-FLUORIMETRY AFTER SYNCHRONIZATION [J].

WATSON, JV ;

TAYLOR, IW .

BRITISH JOURNAL OF CANCER, 1977, 36 (02) :281-287

← 1 2 3 →