ASSAY FOR PLASMA-MEMBRANE PHOSPHATASE-ACTIVITY IN POPULATIONS OF INDIVIDUAL CELLS

被引：10

作者：

WATSON, JV ^{[1
]}

WORKMAN, P ^{[1
]}

CHAMBERS, SH ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,SCH MED,MRC,DEPT RADIOTHERAPEUT,CAMBRIDGE CB2 2QH,ENGLAND

来源：

BIOCHEMICAL PHARMACOLOGY | 1979年 / 28卷 / 06期

关键词：

D O I：

10.1016/0006-2952(79)90364-2

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Flow cytofluorimetric techniques have been used to assay plasma membrane phosphatase activity in populations of individual mouse mammary tumour cells. A new mathematical treatment, involving 'steady-state' kinetic assumptions to analyse the asymptotic portions of the progress curves, gave results which were compatible with normal Michaelian kinetics for the hydrolysis of the fluorogenic substrate 3-O-methyl fluorescein phosphate. The potential application of the methodology in the field of latent anti-neoplastic drugs is discussed. © 1979.

引用

页码：821 / 827

页数：7

共 25 条

[21] DIFFERENTIAL FLUOROCHROMASIA OF HUMAN LYMPHOCYTES AS MEASURED BY FLOW CYTOMETRY [J].

WILDER, ME ;

CRAM, LS .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1977, 25 (07) :888-891

[22] ENZYME ACTIVATED ANTI-TUMOR AGENTS .4. COMPARATIVE KINETICS OF N,N-P-DI-2-CHLOROETHYLAMINOPHENYL PHOSPHATE HYDROLYSIS CATALYZED BY PHOSPHATASES OF NORMAL AND NEOPLASTIC TISSUES [J].

WORKMAN, P ;

DOUBLE, JA .

BIOCHEMICAL PHARMACOLOGY, 1978, 27 (02) :199-202

[23] ENZYME ACTIVATED ANTI-TUMOUR AGENTS .2. ROLE OF ALKALINE-PHOSPHATASE IN RELEASE OF PARA-HYDROXYANILINE MUSTARD FROM ITS PHOSPHATE CONJUGATE IN CELLS IN CULTURE [J].

WORKMAN, P ;

BALL, CR ;

DOUBLE, JA .

BIOCHEMICAL PHARMACOLOGY, 1976, 25 (10) :1139-1144

[24]

WORKMAN P, UNPUBLISHED

[25] INFLUENCE OF TISSUE ENVIRONMENT ON RATES OF METABOLIC PROCESSES AND PROPERTIES OF ENZYMES [J].

YOUDIM, MBH ;

WOODS, HF .

BIOCHEMICAL PHARMACOLOGY, 1975, 24 (03) :317-323

← 1 2 3 →