CHARACTERIZATION OF THE ALPHA-AMYLASE-ENCODING GENE FROM THERMOMONOSPORA-CURVATA

被引:28
作者
PETRICEK, M [1 ]
TICHY, P [1 ]
KUNCOVA, M [1 ]
机构
[1] CZECHOSLOVAK ACAD SCI, INST MICROBIOL, DEPT MOLEC BIOL, VIDENSKA 1083, CS-14220 PRAGUE 4, CZECHOSLOVAKIA
关键词
STREPTOMYCES; PRIMER EXTENSION; RECOMBINANT DNA; MALTOSE INDUCTION; THERMOSTABLE;
D O I
10.1016/0378-1119(92)90305-9
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The nucleotide sequence of a 3007-bp DNA fragment from Thermomonospora curvata CCM3352 containing the coding and regulatory region of the alpha-amylase-encoding gene (tam) was determined. Primer extension mapping was used to determine the 5' end of the transcript, and it was demonstrated that the gene is transcribed from a unique promoter which is also functional in Streptomyces lividans TK24. Transcription of tam in T. curvata was induced by maltose, even in the presence of glucose. In S. lividans, tam was expressed constitutively. The deduced amino acid sequence reveals a considerable similarity with alpha-amylases from streptomycetes.
引用
收藏
页码:77 / 83
页数:7
相关论文
共 38 条
[21]   IMPROVEMENT OF THE DIDEOXY CHAIN TERMINATION METHOD OF DNA SEQUENCING BY USE OF DEOXY-7-DEAZAGUANOSINE TRIPHOSPHATE IN PLACE OF DGTP [J].
MIZUSAWA, S ;
NISHIMURA, S ;
SEELA, F .
NUCLEIC ACIDS RESEARCH, 1986, 14 (03) :1319-1324
[22]  
NAKAJIMA R, 1986, APPL MICROBIOL BIOT, V23, P355
[23]   ISOLATION AND CHARACTERIZATION OF A CIS-ACTING MUTATION CONFERRING CATABOLITE REPRESSION RESISTANCE TO ALPHA-AMYLASE SYNTHESIS IN BACILLUS-SUBTILIS [J].
NICHOLSON, WL ;
CHAMBLISS, GH .
JOURNAL OF BACTERIOLOGY, 1985, 161 (03) :875-881
[24]  
PETRICEK M, 1989, J GEN MICROBIOL, V135, P3303
[25]   ESSENTIAL AND NONESSENTIAL SEQUENCES IN MALPP, A POSITIVELY CONTROLLED PROMOTER IN ESCHERICHIA-COLI [J].
RAIBAUD, O ;
GUTIERREZ, C ;
SCHWARTZ, M .
JOURNAL OF BACTERIOLOGY, 1985, 161 (03) :1201-1208
[26]   MALTOTRIOSE IS THE INDUCER OF THE MALTOSE REGULON OF ESCHERICHIA-COLI [J].
RAIBAUD, O ;
RICHET, E .
JOURNAL OF BACTERIOLOGY, 1987, 169 (07) :3059-3061
[27]   CHANGES IN THE STABILITY OF SPECIFIC MESSENGER-RNA SPECIES IN RESPONSE TO GROWTH STAGE IN BACILLUS-SUBTILIS [J].
RESNEKOV, O ;
RUTBERG, L ;
VONGABAIN, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (21) :8355-8359
[28]   CLONING AND EXPRESSION OF A STREPTOMYCES-FRADIAE NEOMYCIN RESISTANCE GENE IN ESCHERICHIA-COLI [J].
RODGERS, WH ;
SPRINGER, W ;
YOUNG, FE .
GENE, 1982, 18 (02) :133-141
[29]   REGULATORY SEQUENCES INVOLVED IN THE PROMOTION AND TERMINATION OF RNA-TRANSCRIPTION [J].
ROSENBERG, M ;
COURT, D .
ANNUAL REVIEW OF GENETICS, 1979, 13 :319-353
[30]   DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].
SANGER, F ;
NICKLEN, S ;
COULSON, AR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467