CHARACTERIZATION OF THE ALPHA-AMYLASE-ENCODING GENE FROM THERMOMONOSPORA-CURVATA

被引：28

作者：

PETRICEK, M ^{[1
]}

TICHY, P ^{[1
]}

KUNCOVA, M ^{[1
]}

机构：

[1] CZECHOSLOVAK ACAD SCI, INST MICROBIOL, DEPT MOLEC BIOL, VIDENSKA 1083, CS-14220 PRAGUE 4, CZECHOSLOVAKIA

来源：

GENE | 1992年 / 112卷 / 01期

关键词：

STREPTOMYCES; PRIMER EXTENSION; RECOMBINANT DNA; MALTOSE INDUCTION; THERMOSTABLE;

D O I：

10.1016/0378-1119(92)90305-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The nucleotide sequence of a 3007-bp DNA fragment from Thermomonospora curvata CCM3352 containing the coding and regulatory region of the alpha-amylase-encoding gene (tam) was determined. Primer extension mapping was used to determine the 5' end of the transcript, and it was demonstrated that the gene is transcribed from a unique promoter which is also functional in Streptomyces lividans TK24. Transcription of tam in T. curvata was induced by maltose, even in the presence of glucose. In S. lividans, tam was expressed constitutively. The deduced amino acid sequence reveals a considerable similarity with alpha-amylases from streptomycetes.

引用

页码：77 / 83

页数：7

共 38 条

[21] IMPROVEMENT OF THE DIDEOXY CHAIN TERMINATION METHOD OF DNA SEQUENCING BY USE OF DEOXY-7-DEAZAGUANOSINE TRIPHOSPHATE IN PLACE OF DGTP [J].