Transcriptional regulation of Oct4 by a long non-coding RNA antisense to Oct4-pseudogene 5

被引：158

作者：

Hawkins, Peter G. ^{[1
,2
]}

Morris, Kevin, V ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Mol & Expt Med, 10666 N Torrey Pines Rd, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Kellogg Sch Sci & Technol, La Jolla, CA USA

来源：

TRANSCRIPTION-AUSTIN | 2010年 / 1卷 / 03期

关键词：

Oct4; PURA; nucleolin; non-coding RNA; pseudogenes;

D O I：

10.4161/trns.1.3.13332

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Long non-coding RNAs (lncRNAs) have been shown to epigenetically regulate certain genes in human cells. Here we report evidence for the involvement of an antisense lncRNA in the transcriptional regulation of the pluripotency-associated factor Oct4. When an lncRNA antisense to Oct4-pseudogene 5 was suppressed, transcription of Oct4 and Oct4 pseudogenes 4 and 5 was observed to increase. This increase correlated with a loss of silent state epigenetic marks and the histone methyltransferase Ezh2 at the Oct4 promoter. We observed this lncRNA to interact with nucleolin and PURA, a 35 kD single-stranded DNA and RNA binding protein, and found that these proteins may act to negatively regulate this antisense transcript.

引用

页码：165 / 175

页数：11

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