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IDENTIFICATION OF THE GAMMA-2-SUBUNIT PROTEIN IN NATIVE GABA-A RECEPTORS IN BRAIN

被引：17
作者：
BENKE, D
MERTENS, S
TREZCIAK, A
GILLESSEN, D
MOHLER, H
机构：
[1] UNIV ZURICH,INST PHARMACOL,CH-8006 ZURICH,SWITZERLAND
[2] F HOFFMANN LA ROCHE & CO LTD,CENT RES DEPT,CH-4002 BASEL,SWITZERLAND
来源：
EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION | 1990年 / 189卷 / 4-5期
关键词：
GABA-A RECEPTOR; GAMMA-2-SUBUNIT; ANTIPEPTIDE ANTIBODIES;
D O I：
10.1016/0922-4106(90)90127-J
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
GABA(A) receptors with functional benzodiazepine receptors have been reconstituted by coexpression of alpha-, beta- and gamma-subunit cDNAs. In brain, proteins of the alpha- and beta-subunits, but not the gamma-2-subunit have been identified. Using an antipeptide antiserum, we now demonstrate that the gamma-2-subunit is a protein of 43 kDa. It is present in at least 50% of GABA(A) receptors, as shown by immunoprecipitation.
引用
收藏
页码：337 / 340
页数：4
相关论文
共 12 条
[1]  
BENKE D, 1989, J EXP BIOL, V147, P329
[2]   THE NICOTINIC ACETYLCHOLINE-RECEPTOR AND ITS ION CHANNEL [J].
HUCHO, F .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 158 (02) :211-226
[3]  
MALHERBE P, 1990, IN PRESS J NEUROSCI
[4]  
MALHERBE P, 1990, IN PRESS MOL BRAIN R
[5]   GABA-A-RECEPTORS - STRUCTURAL REQUIREMENTS AND SITES OF GENE-EXPRESSION IN MAMMALIAN BRAIN [J].
MOHLER, H ;
MALHERBE, P ;
DRAGUHN, A ;
RICHARDS, JG .
NEUROCHEMICAL RESEARCH, 1990, 15 (02) :199-207
[6]   IMPORTANCE OF A NOVEL GABAA RECEPTOR SUBUNIT FOR BENZODIAZEPINE PHARMACOLOGY [J].
PRITCHETT, DB ;
SONTHEIMER, H ;
SHIVERS, BD ;
YMER, S ;
KETTENMANN, H ;
SCHOFIELD, PR ;
SEEBURG, PH .
NATURE, 1989, 338 (6216) :582-585
[7]   CO-LOCALIZATION OF GABAA RECEPTORS AND BENZODIAZEPINE RECEPTORS IN THE BRAIN SHOWN BY MONOCLONAL-ANTIBODIES [J].
SCHOCH, P ;
RICHARDS, JG ;
HARING, P ;
TAKACS, B ;
STAHLI, C ;
STAEHELIN, T ;
HAEFELY, W ;
MOHLER, H .
NATURE, 1985, 314 (6007) :168-171
[8]   PURIFIED BENZODIAZEPINE RECEPTOR RETAINS MODULATION BY GABA [J].
SCHOCH, P ;
MOHLER, H .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1983, 95 (3-4) :323-324
[9]   THE ROLE OF RECEPTOR SUBTYPE DIVERSITY IN THE CNS [J].
SCHOFIELD, PR ;
SHIVERS, BD ;
SEEBURG, PH .
TRENDS IN NEUROSCIENCES, 1990, 13 (01) :8-11
[10]   2 NOVEL GABAA RECEPTOR SUBUNITS EXIST IN DISTINCT NEURONAL SUBPOPULATIONS [J].
SHIVERS, BD ;
KILLISCH, I ;
SPRENGEL, R ;
SONTHEIMER, H ;
KOHLER, M ;
SCHOFIELD, PR ;
SEEBURG, PH .
NEURON, 1989, 3 (03) :327-337
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