ACID GLYCOHYDROLASE IN CHINESE-HAMSTER (CRICETULUS-GRISEUS) WITH SPONTANEOUS DIABETES .5. SUBCELLULAR-DISTRIBUTION IN THE KIDNEY

1. 1. Seven renal glycohydrolases were measured in four subcellular fractions prepared from highly inbred aglycosuric (AV-line) and glycosuric (XA-line) Chinese hamsters. 2. 2. α-d-galactosidase and β-d-galactosidase were highest in the nuclear (N) and supernatant (S) fractions; both fractions showed reduced activities in the XA animals. 3. 3. α-d-mannosidase was chiefly a particulate enzyme and its decrease in XA animals was evident in N, lysosomal-mitochondrial (LM) and mitochondrial-microsomal (MM) fractions. 4. 4. No significant difference in N-acetyl-β-d-glucosaminidase was found in any of these subcellular fractions between AV and XA animals. 5. 5. Although total α-l-fucosidase and β-d-fucosidase levels were similar in AV and XA kidneys, a difference was observed in the S fraction. 6. 6. β-d-glucuronidase was virtually absent in N and LM fractions and the S fraction of AV kidneys showed higher activity than the XAs. © 1979.