Aptamer-based surface-enhanced Raman scattering detection of ricin in liquid foods

被引:76
作者
He, Lili [1 ]
Lamont, Elise [2 ,3 ]
Veeregowda, Belamaranahally [4 ]
Sreevatsan, Srinand [2 ,3 ]
Haynes, Christy L. [5 ]
Diez-Gonzalez, Francisco [1 ]
Labuza, Theodore P. [1 ]
机构
[1] Univ Minnesota, Dept Food Sci & Nutr, St Paul, MN 55108 USA
[2] Univ Minnesota, Coll Vet Med, Dept Vet Populat Med, St Paul, MN 55108 USA
[3] Univ Minnesota, Coll Vet Med, Dept Vet & Biomed Sci, St Paul, MN 55108 USA
[4] Vet Coll, Dept Vet Microbiol, Bangalore 560024, Karnataka, India
[5] Univ Minnesota, Dept Chem, Minneapolis, MN 55455 USA
关键词
TOXIC PROTEIN; SPECTROSCOPY; PATHOGENS;
D O I
10.1039/c1sc00201e
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A "two-step" aptamer-based surface-enhanced Raman scattering (SERS) detection assay was developed for ricin in liquid foods. Ricin B chain was first captured out of food matrices by aptamer-conjugated silver dendrites and then the spectrum was directly read on the silver dendrites. Aptamer use in this assay promotes ease of manipulation as well as improved sensitivity compared to antibody-based approaches. The limit of detection for ricin B chain was 10 ng mL(-1) in phosphate buffered saline (PBS), 50 ng mL(-1) in orange juice, and 100 ng mL(-1) in milk based on principal component analysis (PCA) of measured spectra. This assay shows great promise as a rapid (< 40 min), sensitive, and simple "Yes/No" method to detect bio-weapons like ricin in liquid foods.
引用
收藏
页码:1579 / 1582
页数:4
相关论文
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