Scanning near-field IR microscopy of proteins in lipid bilayers

被引：17

作者：

Ballout, Fouad ^{[1
]}

Krassen, Henning ^{[2
,3
]}

Kopf, Ilona ^{[1
,4
]}

Ataka, Kenichi ^{[2
,5
]}

Bruendermann, Erik ^{[1
]}

Heberle, Joachim ^{[2
,5
]}

Havenith, Martina ^{[1
]}

机构：

[1] Ruhr Univ Bochum, Dept Phys Chem 2, D-44801 Bochum, Germany

[2] Univ Bielefeld, Dept Chem, D-33615 Bielefeld, Germany

[3] Uppsala Univ, Dept Photochem & Mol Sci, S-75120 Uppsala, Sweden

[4] Trinity Coll Dublin, CRANN, Coll Green, Dublin 2, Ireland

[5] Free Univ Berlin, Dept Phys, D-14195 Berlin, Germany

来源：

PHYSICAL CHEMISTRY CHEMICAL PHYSICS | 2011年 / 13卷 / 48期

关键词：

CYTOCHROME-C-OXIDASE; INFRARED-ABSORPTION SPECTROSCOPY; GOLD ELECTRODE; MEMBRANE RECONSTITUTION; ORIENTED ATTACHMENT; CELLS; RESOLUTION; SURFACES; MONOLAYERS; SCATTERING;

D O I：

10.1039/c1cp21512d

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We use infrared near-field microscopy to chemically map the morphology of biological matrices. The investigated sample is built up from surface-tethered membrane proteins (cytochrome c oxidase) reconstituted in a lipid bilayer. We have carried out infrared near-field measurements in the frequency range between 1600 and 1800 cm(-1). By simultaneously recording the topography and chemical fingerprint of the protein-tethered lipid bilayer with a lateral resolution of 80 nm x 80 nm, we were able to probe locally the chemical signature of this membrane and to provide a local map of its surface morphology.

引用

页码：21432 / 21436

页数：5

共 31 条

[1] Orientational control of the physiological reaction of cytochrome c oxidase tethered to a gold electrode [J].

Ataka, K ;

Richter, B ;

Heberle, J .

JOURNAL OF PHYSICAL CHEMISTRY B, 2006, 110 (18) :9339-9347

[2] Oriented attachment and membrane reconstitution of his-tagged cytochrome c oxidase to a gold electrode:: In situ monitoring by surface-enhanced infrared absorption spectroscopy [J].

Ataka, K ;

Giess, F ;

Knoll, W ;

Naumann, R ;

Haber-Pohlmeier, S ;

Richter, B ;

Heberle, J .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (49) :16199-16206

[3]

Ataka K., 2010, Angewandte Chemie, V122, P5544

[4] Biochemical applications of surface-enhanced infrared absorption spectroscopy [J].

Ataka, Kenichi ;

Heberle, Joachim .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2007, 388 (01) :47-54

[5] SORTING OF GPI-ANCHORED PROTEINS TO GLYCOLIPID-ENRICHED MEMBRANE SUBDOMAINS DURING TRANSPORT TO THE APICAL CELL-SURFACE [J].

BROWN, DA ;

ROSE, JK .

CELL, 1992, 68 (03) :533-544

[6] Cytochrome c oxidase immobilized in stable supported lipid bilayer membranes [J].

Burgess, JD ;

Rhoten, MC ;

Hawkridge, FM .

LANGMUIR, 1998, 14 (09) :2467-2475

[7] Raman spectroscopy and microscopy of individual cells and cellular components [J].

Chan, James ;

Fore, Samantha ;

Wachsman-Hogiu, Sebastian ;

Huser, Thomas .

LASER & PHOTONICS REVIEWS, 2008, 2 (05) :325-349

[8]

CINEK T, 1992, J IMMUNOL, V149, P2262

[9] A STUDY OF CYTOCHROME-C-OXIDASE IN LIPID BILAYER - MEMBRANES ON ELECTRODE SURFACES [J].

CULLISON, JK ;

HAWKRIDGE, FM ;

NAKASHIMA, N ;

YOSHIKAWA, S .

LANGMUIR, 1994, 10 (03) :877-882

[10] A hybrid plasmonic-photonic nanodevice for label-free detection of a few molecules [J].

De Angelis, Francesco ;

Patrini, Maddalena ;

Das, Gobind ;

Maksymov, Ivan ;

Galli, Matteo ;

Businaro, Luca ;

Andreani, Lucio Claudio ;

Di Fabrizio, Enzo .

NANO LETTERS, 2008, 8 (08) :2321-2327

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