微滴式数字聚合酶链式反应在食品安全检测领域的应用

被引:15
作者
牛会敏
王静怡
姚晓洁
魏华琳
陈万胜
邓迎春
机构
[1] 河南省口岸食品检验检测所
关键词
微滴式数字聚合酶链式反应; 绝对定量; 食品安全检测;
D O I
10.19812/j.cnki.jfsq11-5956/ts.2020.24.040
中图分类号
TS207.3 [食品分析与检验];
学科分类号
摘要
微滴式数字聚合酶链式反应(droplet digital polymerase chain reaction, ddPCR)是一种新型核酸扩增技术,可对DNA或RNA分子采用绝对定量的方式进行分析。其结果具有更高的精准度、准确性和灵敏度,大大提升了数字PCR技术的可扩展性与实用性,促进了现代分子生物学在精准定量检测方面的发展和应用。本文重点论述了ddPCR法的技术原理、优势以及在食源性致病微生物定量检测、转基因成分分析、食品源性成分检测等食品安全检测领域的应用研究进展情况。
引用
收藏
页码:9295 / 9300
页数:6
相关论文
共 30 条
  • [11] GB/T 38132-2019. 转基因植物品系定量检测数字PCR法[S]. 2019
  • [12] Species identification and quantification in meat and meat products using droplet digital PCR (ddPCR)[J] . C. Floren,I. Wiedemann,B. Brenig,E. Schütz,J. Beck.Food Chemistry . 2015
  • [13] A microfluidic droplet digital PCR for simultaneous detection of pathogenic Escherichia coli O157 and Listeria monocytogenes[J] . Xiaojun Bian,Fengxiang Jing,Gang Li,Xiaoyun Fan,Chunping Jia,Hongbo Zhou,Qinghui Jin,Jianlong Zhao.Biosensors and Bioelectronics . 2015
  • [14] Assessment of droplet digital PCR for absolute quantification of genetically engineered OXY235 canola and DP305423 soybean samples[J] . Tigst Demeke,Tom Gr?fenhan,Michelle Holigroski,Ursla Fernando,Janice Bamforth,Sung-Jong Lee.Food Control . 2014
  • [15] Quantitative Analysis of Pork and Chicken Products by Droplet Digital PCR[J] . Yicun Cai,Xiang Li,Rong Lv,Jielin Yang,Jian Li,Yuping He,Liangwen Pan,Gerhard Litscher.BioMed Research International . 2014
  • [16] Detection of alpha(0)-thalassemia South-East Asian-type deletion by droplet digital PCR
    Pornprasert, Sakorn
    Prasing, Watcharee
    [J]. EUROPEAN JOURNAL OF HAEMATOLOGY, 2014, 92 (03) : 244 - 248
  • [17] MicroRNA-137 represses Klf4 and Tbx3 during differentiation of mouse embryonic stem cells
    Jiang, Ke
    Ren, Chunyan
    Nair, Venugopalan D.
    [J]. STEM CELL RESEARCH, 2013, 11 (03) : 1299 - 1313
  • [18] Quantification of Zoonotic Bacterial Pathogens within Commercial Poultry Processing Water Samples Using Droplet Digital PCR[J] . Michael J. Rothrock,Kelli L. Hiett,Brian H. Kiepper,Kim Ingram,Arthur Hinton.Advances in Microbiology . 2013 (05)
  • [19] Droplet Digital PCR Measurement of HER2 Copy Number Alteration in Formalin-Fixed Paraffin-Embedded Breast Carcinoma Tissue
    Belgrader, Phillip
    Tanner, Stephanie C.
    Regan, John F.
    Koehler, Ryan
    Hindson, Benjamin J.
    Brown, Alexandra S.
    [J]. CLINICAL CHEMISTRY, 2013, 59 (06) : 991 - 994
  • [20] 转基因大豆MON89788双重数字PCR通用定量检测方法的建立
    刘津
    李婷
    冼钰茵
    吴希阳
    凌莉
    高东微
    [J]. 食品科学 , 2018, (04) : 312 - 319