红曲霉产色素发酵条件优化及抑菌性能的测定

被引:10
作者
后家衡
叶砚
徐晓波
李杰
蒋冬花
机构
[1] 浙江师范大学化学与生命科学学院
基金
浙江省自然科学基金;
关键词
红曲霉; 色素; 优化; 抑菌性能;
D O I
暂无
中图分类号
TQ929 [其他发酵工业];
学科分类号
081703 ; 082203 ;
摘要
优化了3株红曲霉(Mg、M6、M8)产红曲色素的发酵条件:接种量5.0%、培养温度30℃、培养时间72 h、初始pH 6.0。选择产色素最高的Mg菌株,用番薯淀粉培养液进行上罐发酵分析,探讨了Mg菌株液体深层发酵过程中色素产量的变化规律,整个发酵过程分为3个阶段:0~40 h是菌体大量生长阶段,色素产量很低;40~90 h是色素生成期,色素生成稳定且产量高;90 h以后菌体生成和产色素能力下降。色素效价最高达到29.71 U.mL-1。测定了色素对大肠杆菌和金黄色葡萄球菌的抑制作用,当培养液中的色素效价为0.9 U.mL-1时,就开始抑制大肠杆菌和金黄色葡萄球菌生长,色素效价达到3.6 U.mL-1时,其抑菌率也不再增大。
引用
收藏
页码:363 / 367
页数:5
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