液相色谱-串联质谱法对羊肉中鸭肉掺假的鉴别

被引:32
作者
李莹莹 [1 ]
张颖颖 [1 ]
丁小军 [2 ]
李静 [2 ]
赵文涛 [1 ]
陈伟 [2 ]
马燕红 [1 ]
郭文萍 [1 ]
机构
[1] 中国肉类食品综合研究中心
[2] 赛默飞世尔科技
关键词
液相色谱-串联质谱; 肉类掺假; 多肽识别; 鸭肉; 羊肉;
D O I
暂无
中图分类号
O657.63 [质谱分析]; TS251.53 [];
学科分类号
摘要
利用蛋白组学的方法研究了5种常见的食用肉中相对种属特征性多肽,在找到的特征性多肽的基础上,利用液相色谱-质谱联用三重四极杆质谱技术对羊肉与鸭肉混合物种特征性的多肽进行了验证与定量研究。将鸭肉按照1%、5%、10%、20%、50%比例掺加到羊肉中,选择羊肉定量多肽5条,鸭肉定量多肽5条,进行定量分析,线性相关系数为0.99以上,检出限可以达到0.5%。研究表明,利用液相色谱-串联质谱法构建的多肽识别技术可以快速、灵敏、准确地检测出羊肉中的鸭肉成分。
引用
收藏
页码:204 / 209
页数:6
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