不同干预方法对去卵巢骨质疏松大鼠子宫GSK-3β蛋白表达的影响

被引：8

作者：

郭盖

卜淑敏

韩天雨

机构：

[1] 首都体育学院

来源：

中国实验动物学报 | 2014年 / 22卷 / 05期

关键词：

去卵巢大鼠; 全身振动; 跑台运动; 金雀异黄酮; 子宫;

D O I：

暂无

中图分类号：

R580 [];

学科分类号：

摘要：

目的观察全身垂直振动、跑台运动和金雀异黄酮对去卵巢骨质疏松大鼠子宫重量指数和组织形态学以及糖原合成激酶3β(GSK-3β)蛋白表达的影响。方法将72只3月龄雌性SD大鼠按体重分层后随机分为假手术组(12只)和去卵巢组(60只)。去卵巢10周时,将去卵巢组大鼠按体重分层后又随机分为去卵巢组、振动组、跑台组、金雀异黄酮组和雌激素组(每组8～10只),并开始进行不同干预处理。干预处理8周时,于末次处理结束36～48h内,腹主动脉取血处死各组大鼠,用电子天平称量大鼠子宫的重量,用HE染色方法观察子宫形态学的变化,用Western blot方法检测子宫GSK-3β和P-GSK-3β蛋白表达的变化。结果与去卵巢组比较,雌激素组大鼠子宫重量指数显著增加,而振动组、跑台组、金雀异黄酮组大鼠子宫重量指数均无显著变化;与去卵巢组比较,雌激素组、跑台组和振动组大鼠子宫P-GSK-3β/GSK-3β蛋白的比值均显著增加,而金雀异黄酮组无显著变化。结论全身垂直振动和跑台运动均能刺激去卵巢骨质疏松大鼠子宫GSK-3β蛋白的磷酸化,而金雀异黄酮无此效应。

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页码：53 / 58+9 +9

页数：7

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