碳源和Mn2+对地衣芽孢杆菌BacilluslicheniformisWBL-3生产聚γ-谷氨酸的影响

The effects of carbon source and manganese ion on the production of γ -poly (glutamate) ( γ -PGA) was investigated.The results showed that the production of γ -PGA was different on varions carbon sources, and among those, citric acid and glycerol were much better than others.The production of γ -PGA was increased to 15.72?g·L -1 by using the citric acid and glycerol as a combined carbon source.It was also found that the production of γ -PGA could be regulated by control of manganese ion concentration in the medium.Without Mn 2+ ,there was no γ -PGA produced.The production of γ -PGA was increased by increasing Mn 2+ concentration, the optimal concentration of Mn 2+ was 39.0?mg·L -1 .The composition and morphology of γ -PGA were also affected by Mn 2+ .