荧光微球免疫层析技术定量检测河鲀毒素

被引：7

作者：

张世伟

王士峰

姚添琪

杨国武

赖心田

机构：

[1] 深圳市计量质量检测研究院

来源：

食品科学 | 2017年 / 20期

关键词：

河鲀毒素; 亲和位点保护; 荧光微球; 免疫层析;

D O I：

暂无

中图分类号：

TS254.7 [水产制品的标准与检验];

学科分类号：

摘要：

建立河鲀毒素的荧光微球免疫层析检测方法。基于抗体亲和位点保护标记方法制备抗河鲀毒素抗体偶联荧光微球,方法简单稳定,所有结合分离步骤均在亲和柱中完成,避免了亲和位点被破坏而且荧光信号更强,将检测灵敏度提高至原来的8倍左右。所建立的河鲀毒素检测方法IC50为18.4 ng/m L,线性范围为2～200 ng/m L(y=-0.15ln x+0.95,R2=0.98)。检测河鲀样本的最低检出限为10μg/kg,平均相对标准偏差小于25%(n=6)。该方法适用于热加工样品的检测,整个检测过程仅需15 min,且无需使用大型设备,为河鲀餐前速测提供了一定技术手段。

引用

页码：312 / 317

页数：6

共 15 条

[1]

量子点荧光微球免疫层析试纸条定量检测玉米中赭曲霉毒素A[J]. 周耀锋,熊斯诚,江湖,段宏,熊勇华,Andrew Wang.分析化学. 2015(12)

[2] 基于不同载体免疫原制备河豚毒素抗体的研究 [J].

刘燕婷 ;

钟青萍 ;

雷红涛 ;

张挺 ;

余海虎 ;

孙远明 .

卫生研究, 2008, (02) :234-236

[3]

An ultra-sensitive monoclonal antibody-based fluorescent microsphere immunochromatographic test strip assay for detecting aflatoxin M 1 in milk[J] . Xiya Zhang,Kai Wen,Zhanhui Wang,Haiyang Jiang,Ross C. Beier,Jianzhong Shen.Food Control . 2016

[4] Tetrodotoxin detection and species identification of pufferfish in retail roasted fish fillet by DNA barcoding in China [J].

Li, Nan ;

Shen, Qing ;

Wang, Jiahui ;

Han, Chunhui ;

Ji, Rong ;

Li, Fengqin ;

Jiang, Tao .

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2015, 32 (12) :2148-2153

[5]

Development of ELISA and colloidal gold immunoassay for tetrodotoxin detetcion based on monoclonal antibody[J] . Sumei Ling,Qing-Ai Chen,Yuming Zhang,Rongzhi Wang,Ni Jin,Jie Pang,Shihua Wang.Biosensors and Bioelectronics . 2015

[6]

A rapid method for tetrodotoxin (TTX) determination by LC-MS/MS from small volumes of human serum, and confirmation of pufferfish poisoning by TTX monitoring[J] . Kazunari Tsujimura,Kimiko Yamanouchi.Food Additives & Contaminants: Part A . 2015 (6)

[7]

Development of standardized methodology for identifying toxins in clinical samples and fish species associated with tetrodotoxin-borne poisoning incidents[J] . Tai-Yuan Chen,Cheng-Hong Hsieh,Deng-Fwu Hwang.Journal of Food and Drug Analysis . 2015

[8]

Mechanism of tetrodotoxin block and resistance in sodium channels[J] . Rong Chen,Shin-Ho Chung.Biochemical and Biophysical Research Communications . 2014

[9] Development of a lateral flow fluorescent microsphere immunoassay for the determination of sulfamethazine in milk [J].

Chen, Rui ;

Li, Heng ;

Zhang, Han ;

Zhang, Suxia ;

Shi, Weimin ;

Shen, Jianzhong ;

Wang, Zhanhui .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2013, 405 (21) :6783-6789

[10]

Development of a multiplexed fluorescent immunoassay for the quantitation of antibody responses to four Neisseria meningitidis serogroups[J] . Thomas B. Martins,Troy D. Jaskowski,Anne Tebo,Harry R. Hill.Journal of Immunological Methods . 2008 (1)

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