短小芽孢杆菌A-30耐碱性木聚糖酶的纯化及性质研究

被引：19

作者：

陈士成

曲音波

刘相梅

高培基

机构：

[1] 山东大学微生物技术国家重点实验室!济南,山东大学微生物技术国家重点实验室!济南,山东大学微生物技术国家重点实验室!济南,山东大学微生物技术国家重点实验室!济南

来源：

中国生物化学与分子生物学报 | 2000年 / 05期

关键词：

Bacillus pumilus; Xylanase; Purification; Alkali tolerant;

D O I：

10.13865/j.cnki.cjbmb.2000.05.027

中图分类号：

Q814 [酶工程];

学科分类号：

082203 ;

摘要：

The extracellular xylanase from the crude culture filtration of Bacillus pumilus A 30 was purified by ammonium sulfate fractionation,CM Sephadex C 50,DEAE Sephadex A 50 and Bio gel P 30 chromatographies.SDS PAGE analysis indicated that the xylanase had molecular weight 23 kD.Its isoelectric point p I 8.5 was determined by IEF PAGE.Its temperature optima for the activity and its optimum pH was 50℃ and 7.5 respectively.It was stable in a broad pH range and showed good stability below 50℃。Its activity was strongly inhibited by Ag +,Cu 2+ ,Fe 3+ ,Co 2+ ,Hg 2+ ,Zn 2+ ,Al 3+ and increased dramatically by Mg 2+ ,Ca 2+ .Its K m and V m were 7.42 mg·ml -1 and 654 μmol·min -1 ·mg -1 respectively.

引用

页码：698 / 701

页数：4

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应用与环境生物学报 , 1999, (04) :71-77

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