胃癌患者脾胃虚弱证形成与舌苔菌群相关性研究

被引：25

作者：

沈睿 ^{[1
]}

李美凤 ^{[1
]}

彭宇恒 ^{[1
]}

滕钰浩 ^{[2
]}

钱峻 ^{[3
]}

董伟 ^{[1
]}

詹瑧 ^{[1
]}

张军峰 ^{[1
]}

机构：

[1] 南京中医药大学医学院·整合医学学院

[2] 南京中医药大学附属医院

[3] 南京中医药大学中医学院·中西医结合学院

来源：

南京中医药大学学报 | 2020年 / 36卷 / 02期

关键词：

胃癌; 脾胃虚弱证; 舌苔菌群; 系统生物学;

D O I：

10.14148/j.issn.1672-0482.2020.0221

中图分类号：

R273 [中医肿瘤科];

学科分类号：

100502 [中医临床基础];

摘要：

目的探讨胃癌患者脾胃虚弱证形成的系统生物学机制。方法招募22例胃癌脾胃虚弱证患者和32例体检者作为对照,基于16S rDNA和18S rDNA基因高通量测序分析舌苔细菌和真菌,电化学发光技术检测血清20种炎症因子,超高效液相色谱质谱联用技术(UPLC-MS)分析血清代谢组学。结果与对照组相比,脾胃虚弱证患者舌苔厚壁菌门相对丰度显著升高,而拟杆菌门、变形菌门、梭杆菌门相对丰度降低(P<0.05)。线性判别分析(LDA)发现,芽孢杆菌属、肠球菌属、乳球菌属、沙雷氏菌属是胃癌脾胃虚弱证患者舌苔标志菌属。与对照组相比,胃癌脾胃虚弱证患者血清GM-CSF、IL-17α、IL-12/IL23P40和IL-6水平显著升高(P<0.05),而IL-5、TNF-β、IL-4水平显著降低(P<0.05),9种血清代谢分子(L-赖氨酸、二十碳五烯酸、L-天冬酰胺、溶血磷脂酰乙醇胺、磷脂酸、亚油酸、9-十六碳烯酸、α-亚麻酸和L-苯丙氨酸)显著上升(P<0.05)。Spearman相关性分析,IL-6、TNF-β、IL-5与卟啉单胞菌属、奈瑟菌属均显著相关(P<0.05),奈瑟菌属、梭杆菌属与9种血清代谢分子均显著负相关(P<0.05)。结论胃癌脾胃虚弱证患者舌苔菌群结构差异与血清炎症因子和代谢组学相关,为阐明脾胃虚弱证的形成机制提供新的思路与方法。

引用

页码：221 / 228

页数：8

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