共 3 条
[3]
生物化学实验原理和方法.[M].李建武等编;.北京大学出版社.1994,
L-谷氨酰胺高产菌株的定向选育及产胺条件的研究
被引：9
作者：
汪世华
白文钊
吴思方
张先恩
机构：
[1] 湖北工学院生物工程系
[2] 陕西理工学院生物系
[3] 中国科学院武汉病毒研究所
来源：
关键词：
谷氨酰胺;
诱变育种;
发酵;
D O I：
10.13343/j.cnki.wsxb.2002.06.018
中图分类号：
Q939.9 [应用微生物学];
学科分类号：
摘要：
Original strain,Corynebacteriun glutamicum S9114,was mutated by γ-radiation and DES.Then a strain SH-44,producing Glutamine,was obtained.Fermentatin was conducted in 500mL flask,each containing 30mL of medium consisted of 18% glucose,4% NH -4Cl,1.4% urea,0.5% cornsteepliquor,0.05%K -2HPO,0.05%KH -2PO,0.05%MgSO -4,0.0005%Fe 2+,0.0025% Mn 2+,0.0005% Zn 2+ and 5% CaCO 3.The optima culture condition were as below:initial pH7.0,34℃,shaking speed 300r/min,and cultivation time 72 d.Under optimum condition,SH-44 was able to accumulate an average of 55.3 mg/mL and the highest of 55.8mg/ml of L-glutamine.
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页码：751 / 754
页数：4
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