NMR联用HILIC-UPLC-MS/MS测定TNBS诱导的结肠炎大鼠尿液和血浆代谢物变化

被引：3

作者：

刘雯 ^{[1
]}

孙央雯 ^{[1
]}

邹宝荣 ^{[1
]}

肖丹 ^{[1
]}

蔡颖 ^{[1
]}

刘德亮 ^{[1
]}

廖琼峰 ^{[2
]}

谢智勇 ^{[1
]}

机构：

[1] 中山大学药学院(深圳)

[2] 广州中医药大学中药学院

来源：

中山大学学报(医学科学版) | 2021年 / 42卷 / 04期

基金：

广州市科技计划项目;

关键词：

结肠炎; 代谢组学; 核磁共振; HILIC模式超高效液相色谱串联三重四级杆质谱法;

D O I：

暂无

中图分类号：

R574.62 [结肠疾病]; R-332 [医用实验动物学];

学科分类号：

100201 [内科学]; 100402 [劳动卫生与环境卫生学];

摘要：

【目的】基于非靶标和靶标代谢组学技术,探讨三硝基苯磺酸(TNBS)诱导的结肠炎模型大鼠尿液以及血浆中内源性代谢物的变化。【方法】16只雄性SD大鼠随机分成对照组和模型组,每组8只,对模型组大鼠给予TNBS灌肠以建立实验性大鼠结肠炎模型。利用核磁共振氢谱(1H NMR)检测两组大鼠尿液代谢物,同时联用HILIC模式超高效液相色谱串联三重四级杆质谱(HILIC-UPLC-MS/MS)测定以上大鼠尿液以及血浆中氨基酸水平,并通过多元变量统计方法对代谢组学数据进行分析。【结果】同对照组相比,1H NMR结果表明模型组大鼠尿液代谢轮廓发生明显改变,其中丙酮酸、甲酸、甲胺与柠檬酸水平升高,氧化三甲胺和丙二酸水平降低(均P <0.05);HILICUPLC-MS/MS结果显示模型组大鼠尿液中苯丙氨酸、组氨酸水平显著上升,血浆中赖氨酸、精氨酸、苯丙氨酸、亮氨酸、甘氨酸、色氨酸、脯氨酸、组氨酸和酪氨酸水平显著升高,但谷氨酰胺、缬氨酸、丙氨酸和异亮氨酸水平明显降低(均P <0.05)。【结论】经TNBS诱导的结肠炎模型大鼠,其能量代谢、胺类代谢以及氨基酸代谢通路受到影响;采用的1H NMR与HILIC-UPLC-MS/MS联用的多元代谢组学方法,揭示了结肠炎大鼠尿液及血浆代谢物的变化,为炎症性肠病的机制研究与相关生物标志物的探索提供了新的思路。

引用

收藏

页码：504 / 512

页数：9

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