镉胁迫下萝卜基因组DNA甲基化敏感扩增多态性分析

被引：145

作者：

杨金兰

柳李旺

龚义勤

黄丹琼

王峰

何玲莉

机构：

[1] 南京农业大学园艺学院作物遗传与种质创新国家重点实验室

来源：

植物生理与分子生物学学报 | 2007年 / 03期

关键词：

萝卜; 镉胁迫; DNA甲基化; 甲基化敏感扩增多态性;

D O I：

暂无

中图分类号：

X173 [环境植物学];

学科分类号：

083001 [环境科学];

摘要：

应用甲基化敏感扩增多态性（MSAP）技术分析了重金属镉（cd）胁迫处理后萝卜基因组DNA甲基化程度的变化。结果表明,经50、250和500mg/L CdCl2处理后,MSAP比率分别为37%、43%和51%,均高于对照（34%）;全甲基化率（双链CmCGG）分别为23%、25%和27%,而其对照为22%,表明重金属CdCl2胁迫后,某些位点发生了重新甲基化。萝卜叶片DNA中总甲基化水平的增加与CdCl2处理浓度呈显著正相关。甲基化变异可分为重新甲基化、去甲基化、不定类型以及与对照相同的甲基化模式等类型,Cd胁迫处理引起的植株基因组DNA甲基化程度的提高主要是重新甲基化。

引用

页码：219 / 226

页数：8

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