黄芩苷通过Wnt/β-catenin信号通路对大鼠骨髓间充质干细胞成骨分化的促进作用

被引：39

作者：

李晨睿

孟志远

牛银波

翟远坤

潘亚磊

谢丽

梅其炳

机构：

[1] 西北工业大学生命学院空间生物实验模拟技术国防重点学科实验室

来源：

中国药理学通报 | 2015年 / 31卷 / 07期

基金：

中央高校基本科研业务费专项资金资助;

关键词：

黄芩苷; 大鼠骨髓间充质干细胞; 成骨分化; Wnt/β-catenin信号通路; 碱性磷酸酶;

D O I：

暂无

中图分类号：

R285 [中药药理学];

学科分类号：

100806 [中药药理学];

摘要：

目的研究黄芩苷(baicalin)对大鼠骨髓间充质干细胞(rat bone marrow derived mesenchymal stem cells,r BMSC)成骨分化过程中Wnt/β-catenin信号通路的影响。方法采用贴壁筛选法体外培养r BMSC,给予黄芩苷3、5、7 d后,比较药物处理组与对照组之间碱性磷酸酶(ALP)的活性。同时检测给予黄芩苷对碱性磷酸酶阳性克隆和矿化结节形成的影响。提取总mRNA和总蛋白,用实时荧光定量PCR检测黄芩苷对Wnt10a、GSK-3β、β-catenin以及LEF1 mRNA水平的影响。免疫印迹检测药物处理对β-catenin以及Runx2蛋白表达量的影响。结果黄芩苷明显提高ALP的活性。10μmol·L-1浓度的黄芩苷还可增加碱性磷酸酶克隆数和钙化结节的形成。黄芩苷还可提高Wnt10a、β-catenin、GSK-3β、LEF1以及osteocalcin的mRNA水平,并提高β-catenin和Runx2的蛋白表达量。结论黄芩苷在0.1～50μmol·L-1的给药浓度下可促进rB MSC的成骨分化成熟,Wnt/β-catenin信号可能参与调控rB MSC的成骨分化。

引用

页码：919 / 924

页数：6

共 12 条

[1]

Adverse Effects of Osteocytic Constitutive Activation of ?‐Catenin on Bone Strength and Bone Growth.[J].Sixu Chen;Jianquan Feng;Quanwei Bao;Ang Li;Bo Zhang;Yue Shen;Yufeng Zhao;Qingshan Guo;Junjun Jing;Shuxian Lin;Zhaowen Zong.J Bone Miner Res.2015, 7

[2]

Coordinated regulation of mesenchymal stem cell differentiation on microstructured titanium surfaces by endogenous bone morphogenetic proteins.[J].Rene Olivares-Navarrete;Sharon L. Hyzy;David A. Haithcock;Caitlin A. Cundiff;Zvi Schwartz;Barbara D. Boyan.Bone.2015,

[3]

Characterization of bone marrow-derived mesenchymal stem cells in aging.[J].Natasha Baker;Lisa B. Boyette;Rocky S. Tuan.Bone.2015,

[4]

Effects of BMP-2 and FGF2 on the Osteogenesis of Bone Marrow-Derived Mesenchymal Stem Cells in Hindlimb-Unloaded Rats [J].

Qian, Xiaodan ;

Zhang, Cui ;

Chen, Guojun ;

Tang, Zihua ;

Liu, Quanwen ;

Chen, Jiarong ;

Tong, Xiangming ;

Wang, Jinfu .

CELL BIOCHEMISTRY AND BIOPHYSICS, 2014, 70 (02) :1127-1136

[5]

Comparative Effects of Hispidulin, Genistein, and Icariin with Estrogen on Bone Tissue in Ovariectomized Rats [J].

Yang, Lijuan ;

Yu, Zixu ;

Qu, Haili ;

Li, Minmin .

CELL BIOCHEMISTRY AND BIOPHYSICS, 2014, 70 (01) :485-490

[6]

The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone Resorption In Vitro.[J].Yuan-Kun Zhai;Ya-Lei Pan;Yin-Bo Niu;Chen-Rui Li;Xiang-Long Wu;Wu-Tu Fan;Ting-Li Lu;Qi-Bing Mei;Cory J. Xian;Iacopo Chiodini.International Journal of Endocrinology.2014,

[7]

Wnt6; Wnt10a and Wnt10b inhibit adipogenesis and stimulate osteoblastogenesis through a β-catenin-dependent mechanism.[J]..Bone.2011, 2

[8]

Orally bioavailable GSK-3α/β dual inhibitor increases markers of cellular differentiation in vitro and bone mass in vivo [J].