游泳运动对高脂引起的大鼠非酒精性脂肪肝的预防作用及机制

被引：6

作者：

傅建 ^{[1
]}

齐洁 ^{[2
]}

张波 ^{[2
]}

张钧 ^{[2
]}

机构：

[1] 扬州大学体育学院

[2] 上海师范大学体育学院

来源：

体育与科学 | 2019年 / 40卷 / 03期

关键词：

非酒精性脂肪肝; 游泳; 高脂饮食; AMPKα; PPARα; AKT;

D O I：

10.13598/j.issn1004-4590.2019.03.016

中图分类号：

R87 [运动医学];

学科分类号：

100216 ;

摘要：

背景与目的:非酒精性脂肪肝病(non-alcoholic fatty liver disease,NAFLD)在人群中的患病率仍在逐年增加,引起了巨大的健康问题。已知运动有助于NAFLD的治疗,但运动方式与不同运动方案(运动的类型、时长与强度)对NAFLD的预防效果以及相关机制的研究目前还相当有限。本研究旨在探讨游泳运动对高脂饮食所诱导的大鼠NAFLD的预防作用及其机制。材料与方法:以6周龄Sprague-Dawley(SD)纯系雄性大鼠为实验动物,随机分为4组:普通饲料对照组(CON组)、高脂饮食NAFLD组(HFD组)、高脂饮食+60min运动预防组(HE60组)、高脂饮食+90min运动预防组(HE90组),持续12周。通过体重、肝重、肝功指标、血脂和肝脂的测定以及HE染色分析评价运动对NAFLD的预防作用。通过免疫印迹和定量PCR测定关键信号通路上基因的蛋白质和mRNA表达水平,揭示运动预防NAFLD的分子机制。结果:60 min和90 min的游泳运动可以显著抑制高脂饮食引起的大鼠体重和肝重的增加,抑制高脂饮食引起的大鼠血脂、ALT和AST的升高,以及抑制高脂饮食引起的大鼠肝脏脂肪变性。与60 min游泳运动相比,90 min降低体重更为明显,对NAFLD的预防作用也更有效。在预防机制方面,运动可提高肝脏中Akt的磷酸化水平,增加肝脏中AMPKα和PPARα的表达,抑制SREBP-1c和SCD1基因的表达。结论:游泳运动对高脂引起的大鼠NAFLD具有预防作用。AMPKα和PPARα在这种预防作用中扮演重要角色,是潜在的分子靶点。此外,本研究还为确立合适的运动方案预防NAFLD提供了参考信息。

引用

页码：108 / 115

页数：8

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