西江流域地表水微生物污染定量源解析

被引：4

作者：

张杨 ^{[1
,2
]}

吴仁人 ^{[2
,3
]}

杨戈 ^{[4
]}

汪光 ^{[2
,3
]}

王一舒 ^{[2
,3
]}

林凯荣 ^{[1
]}

李开明 ^{[2
,3
]}

钟杰 ^{[5
]}

机构：

[1] 中山大学水资源与环境系

[2] 环境保护部华南环境科学研究所

[3] 广东省水与大气污染防治重点实验室

[4] 肇庆市环境保护监测站

[5] 仲恺农业工程学院环境科学与工程学院

来源：

中国环境科学 | 2018年 / 10期

基金：

广东省自然科学基金;

关键词：

微生物污染; 源强; 定量; 西江;

D O I：

10.19674/j.cnki.issn1000-6923.2018.0426

中图分类号：

X522 [河流];

学科分类号：

摘要：

水体微生物污染标记物的源强特征是定量解析微生物污染来源的关键.应用实时荧光定量PCR(qPCR)技术分别对人源(qHS601F/qBac725R)、牛源(BacB2-590F/Bac708Rm)、猪源(Bac41F/Bac163R)及禽类(qC160F-HU/qBac265R-HU)特异性拟杆菌引物的源强特征进行了探索,并进一步对西江流域广东段进行微生物污染来源定量解析,结果表明:针对不同目标宿主,不同特异性拟杆菌引物的源强特征为人源(qHB)>猪源(qPB)>牛源(qRB)>禽类(qCB),DNA模板浓度对引物的源强特征影响较小.选取的目标研究区域水体普遍受到人、猪、牛及禽类粪便污染.干流中qHB、qCB及qPB自上游至下游缓慢升高.各支流中qHB、qCB及qPB的平均浓度较干流均上升了1个数量级,qRB在不同河段变化较小.说明下游河段的生活污水、猪源及禽类粪便污染较为严重,牛源污染的影响较小.统计分析显示在污染水平较高的各支流中,人源特异性拟杆菌引物与传统指示菌具有一定的相关性(P<0.05),说明生活污水是传统指示菌浓度较高的主要原因,且为持续排放源.

引用

页码：3889 / 3896

页数：8

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