发酵冬虫夏草菌丝体中虫草多糖含量的检测及结构鉴定

被引：20

作者：

许峰 ^{[1
]}

吴玲芳 ^{[1
]}

林善 ^{[2
]}

王鸿艳 ^{[1
]}

滕毅 ^{[1
]}

柳志强 ^{[2
]}

郑裕国 ^{[2
]}

机构：

[1] 杭州中美华东制药有限公司

[2] 浙江工业大学生物工程研究所

来源：

基因组学与应用生物学 | 2014年 / 33卷 / 06期

关键词：

冬虫夏草; 虫草多糖; 分离提取; 含量测定; 结构表征;

D O I：

10.13417/j.gab.033.001294

中图分类号：

S567.35 [];

学科分类号：

摘要：

冬虫夏草是一种中国传统的真菌药材,水溶性虫草多糖作为冬虫夏草菌丝体中的一种重要活性成分,有其独特的生物学活性,利用超声提取法可以分离得到虫草多糖。本研究通过采用苯酚-硫酸法测得中国被毛孢中多糖含量为3.848%。经由热水提取、乙醇沉淀后,通过DEAE-Sephadex A-25离子交换柱对中国被毛孢胞外多糖进行了分级,Sephacryl S-100 HR层析柱进一步分离多糖,得到中性多糖HSP-1以及酸性多糖HSP-2,其分子量分别为3.5伊103k D和3.4伊103k D。结合酸水解、乙酰化、GC-MS、FT-IR、1H NMR以及13C NMR等方法对两个组分的结构进行表征。GC-MS检测确定HSP-1和HSP-2的单糖组成为甘露糖、半乳糖和葡萄糖,摩尔比分别为0.34:0.38:1及0.28:0.24:1。红外光谱和核磁共振的结果表明,HSP-1和HSP-2主要由β-糖苷键连接,HSP-1含有少量α-糖苷键。

引用

页码：1294 / 1302

页数：9

共 23 条

[1]

Effects of extraction methods on antioxidant activities of polysaccharides from camellia seed cake [J].

Shen, Shian ;

Cheng, Haoran ;

Li, Xu ;

Li, Tian ;

Yuan, Ming ;

Zhou, Yonghong ;

Ding, Chunbang .

EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2014, 238 (06) :1015-1021

[2]

Structural characterization and antioxidant properties of polysaccharides from two <Emphasis Type="Italic">Schisandra</Emphasis> fruits.[J].Shuhan Chen;Haixia Chen;Zhaoshuai Wang;Jingge Tian;Jia Wang.European Food Research and Technology.2013, 5

[3]

Structure elucidation and immunological activity of a novel pectic polysaccharide from the stems of Avicennia marina [J].

Fang, Xubo ;

Chen, Xiaoe .

EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2013, 236 (02) :243-248

[4]

Comparison of the structural characterization and biological activity of acidic polysaccharides from Cordyceps militaris cultured with different media.[J].Fengyao Wu;Hui Yan;Xiaoning Ma;Junqiang Jia;Guozheng Zhang;Xijie Guo;Zhongzheng Gui.World Journal of Microbiology and Biotechnology.2012, 5

[5]

Structural elucidation of an exopolysaccharide from mycelial fermentation of a Tolypocladium sp. fungus isolated from wild Cordyceps sinensis.[J].Jing-Kun Yan;Lin Li;Zhao-Mei Wang;Jian-Yong Wu.Carbohydrate Polymers.2009, 1

[6]

Determination of nucleosides and nucleobases in different species of Cordyceps by capillary electrophoresis–mass spectrometry.[J].Feng-Qing Yang;Liya Ge;Jean Wan Hong Yong;Swee Ngin Tan;Shao-Ping Li.Journal of Pharmaceutical and Biomedical Analysis.2009, 3

[7]

Isolation, chemical characterization and antioxidant activities of two polysaccharides from the gel and the skin of Aloe barbadensis Miller irrigated with sea water [J].

Liu Chun-hui ;

Wang Chang-hai ;

Xu Zhi-liang ;

Wang Yi .

PROCESS BIOCHEMISTRY, 2007, 42 (06) :961-970

[8]

Alternative medium for production of Pleurotus ostreatus biomass and potential antitumor polysaccharides.[J].Regina Maria Miranda Gern;Elisabeth Wisbeck;Jamile Rosa Rampinelli;Jorge Luiz Ninow;Sandra Aparecida Furlan.Bioresource Technology.2007, 1

[9]

Studies on isolation and structural features of a polysaccharide from the mycelium of an Chinese edible fungus (Cordyceps sinensis) [J].

Wu, YL ;

Sun, CR ;

Pan, YJ .

CARBOHYDRATE POLYMERS, 2006, 63 (02) :251-256

[10]

Enhanced simultaneous production of mycelia and intracellular polysaccharide in submerged cultivation of Cordyceps jiangxiensis using desirability functions [J].

Xiao, Jian-hui ;

Chen, Dai-xiong ;

Wan, Wei-hong ;

Hu, Xi-jie ;

Qi, Ying ;

Liang, Zong-qi .

PROCESS BIOCHEMISTRY, 2006, 41 (08) :1887-1893

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