微滴式数字PCR技术进展

被引：16

作者：

王琦

范颖

机构：

[1] 首都医科大学附属北京地坛医院肝病中心

来源：

中国医学前沿杂志(电子版) | 2016年 / 11期

关键词：

PCR; RNA; 核酸检测; 绝对定量; 微滴; 实时荧光;

D O I：

暂无

中图分类号：

R440 [];

学科分类号：

摘要：

<正>1985年,美国科学家Kary Mullis发明了聚合酶链反应(polymerase chain reaction,PCR)。PCR是现代生命科学研究中最常规的实验方法之一,其发展经历了3个主要阶段:1操作烦琐、只适用于定性研究的琼脂糖电泳PCR;2实时荧光定量PCR,也是目前应用最广泛的PCR技术;3数字PCR(digital PCR,d PCR),属于第3代PCR,最

引用

页码：15 / 19

页数：5

共 9 条

[1] 数字PCR技术的发展和应用
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[7] Detection of BRAF-V600E and V600K in melanoma circulating tumour cells by droplet digital PCR[J] . Anna L. Reid,James B. Freeman,Michael Millward,Melanie Ziman,Elin S. Gray.Clinical Biochemistry . 2014
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[9] Tolerance of Droplet-Digital PCR vs Real-Time Quantitative PCR to Inhibitory Substances
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