基于高分辨率熔解曲线技术快速筛选黄瓜SNP1

被引:7
作者
兰青阔 [1 ]
张桂华 [2 ]
王永 [1 ]
赵新 [1 ]
朱珠 [1 ]
孙玉河 [2 ]
郭永泽 [1 ]
程奕 [1 ]
机构
[1] 天津市农业科学院中心实验室
[2] 天津科润农业科技股份有限公司黄瓜研究所
关键词
黄瓜; SNP标记; 高分辨率熔解曲线;
D O I
暂无
中图分类号
S642.2 [黄瓜];
学科分类号
摘要
为筛选用以黄瓜纯度检验和品种鉴定的SNP位点,以17个黄瓜杂交品种及其34份亲本为SNP筛选群体,利用高分辨率熔解曲线(high resolution melting,HRM)技术筛选SNP位点。本研究通过HRM分析和焦磷酸测序确认,获得CLA0(TT/--)、CLA1(C/T)和CLA6(A/G)三个SNP位点,群体中多态信息量(PIC)分别为0.549、0.366和0.477;并对市售12个杂交黄瓜种分型鉴定,结果表明,三个SNP位点适用于其中11个市售品种的纯度鉴定,具备用于品种鉴定的潜能。
引用
收藏
页码:642 / 647
页数:6
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