OsBTF3转基因水稻对病原菌侵染的反应和防卫基因的表达分析

被引:6
作者
李广旭 [1 ,2 ]
陈华民 [1 ]
吴茂森 [1 ]
何晨阳 [1 ]
机构
[1] 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室
[2] 辽宁省农业科学院果树研究所
关键词
水稻; OsBTF3; 转基因; 感病性; 防卫基因表达;
D O I
暂无
中图分类号
S435.111.4 [侵(传)染性病害];
学科分类号
090401 [植物病理学];
摘要
为了阐明OsBTF3基因在水稻对白叶枯病菌(Xanthomonas oryzae pv.oryzae,Xoo)和细菌性条斑病菌(X.oryzae pv.oryzicola,Xoc)侵染抗/感反应中的功能,本研究以OsBTF3过量表达和RNAi转基因株系为材料,比较分析了T1代株系对病菌侵染的抗感病反应,定量测定了防卫基因的表达动态。结果表明,与野生型对照株相比,无论过量表达株系,还是RNAi株系对Xoo和Xoc侵染均表现为更加感病的反应;经Xoo接种后,过量表达和RNAi株系OE37和RNAi株系RI30防卫基因的表达发生了不同程度的变化。因此,OsBTF3增量/减量表达对水稻抗/感病性具有重要的调控作用,这种作用可能并不依赖于水杨酸(SA)和茉莉酸(JA)介导的信号途径。
引用
收藏
页码:169 / 175
页数:7
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