Identification of rice (Oryza sativa) proteins linked to the cyclin-mediated regulation of the cell cycle

被引:23
作者
Cooper, B [1 ]
Hutchison, D [1 ]
Park, S [1 ]
Guimil, S [1 ]
Luginbühl, P [1 ]
Ellero, C [1 ]
Goff, SA [1 ]
Glazebrook, J [1 ]
机构
[1] Torrey Mesa Res Inst, San Diego, CA 92121 USA
关键词
cell cycle; mitosis; plants; protein interactions; yeast two-hybrid assay; CALMODULIN-BINDING PROTEIN; GENES; TRANSCRIPTION; DIFFERENTIATION; ARABIDOPSIS; EXPRESSION; GROWTH; KINASE; CDC2;
D O I
10.1023/B:PLAN.0000007001.30865.0f
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Yeast two-hybrid assays were used to identify rice proteins interacting with two rice cyclins and other proteins potentially involved in cell cycling. The DNA sequences encoding 119 protein fragments identified were then compared by BLAST against proteins in GenBank. The proteins found include myosin-like proteins, transcription factors, kinesins, centromere proteins and undefined proteins. Based on interactions with cyclins and other elements required for cycling, we believe the undefined proteins may be involved in associated cycling processes. The identification of proteins involved in cell cycle regulation in rice may allow for the control of agronomic traits involving plant growth or development.
引用
收藏
页码:273 / 279
页数:7
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