红豆草耐盐愈伤组织的筛选及植株再生(英文)

被引：9

作者：

徐云远

王鸣刚

贾敬芬

机构：

[1] 中国科学院植物研究所!北京

[2] 兰州大学生物系!兰州

来源：

西北植物学报 | 2000年 / 01期

关键词：

体外筛选; 红豆草; 植株再生; 耐盐性;

D O I：

暂无

中图分类号：

Q945.51 [无性繁殖、孢子繁殖];

学科分类号：

090102 ;

摘要：

将红豆草种子在含１．２％ＮａＣｌ的ＭＳ培养基上萌发以消除盐敏感的幼苗，把存活的幼苗下胚轴切段在含１ｍｇ／Ｌ２，４－Ｄ、０．５ｍｇ／Ｌ６－ＢＡ及１．２％ＮａＣｌ的ＭＳ培养基上诱导愈伤组织。通过连续筛选得到可耐受１．８％ＮａＣｌ的愈伤组织。在有０．２ｍｇ／ＬＮＡＡ和１ｍｇ／ＬＩＡＡ存在下该愈伤组织分化出芽，待幼苗长至３ｃｍ左右时转到含２ｍｇ／ＬＮＡＡ或ＩＢＡ的１／２ＭＳ培养基上生根。与对照愈伤组织相比，所筛选的愈伤组织以及自再生植株诱导的愈伤组织均表现出对渗透胁迫的较高耐性

引用

页码：15 / 21

页数：7

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