乳酸乳球菌在疫苗递呈载体中的应用

被引：5

作者：

刘晓锐 ^{[1
]}

张晓红 ^{[2
]}

吴洁 ^{[1
]}

机构：

[1] 中国药科大学生命科学与技术学院微基因药学实验室

[2] 南京医科大学南京市妇幼保健院生殖医学国家重点实验室

来源：

药物生物技术 | 2014年 / 21卷 / 01期

关键词：

乳酸乳球菌; 疫苗; 抗原; 表达; 粘膜免疫; 载体;

D O I：

10.19526/j.cnki.1005-8915.2014.01.017

中图分类号：

R915 [药物生物学];

学科分类号：

摘要：

乳酸菌是人和动物肠道内的常见细菌,被公认为安全级微生物。近年来,对于乳酸菌作为宿主菌表达外源蛋白或抗原的研究取得了一定进展。乳酸乳球菌(Lactococcus lactis)是乳酸菌的模式菌种,其基因组测序已经完成,因而方便研究者根据实际需要利用分子生物学工具将其改造为各种表达载体可用的受体菌。目前已经开发了一系列质粒型克隆载体和整合型克隆载体系统及蛋白定位系统,已有多种病原微生物抗原成功在乳酸乳球菌中表达,重组乳酸乳球菌作为粘膜免疫疫苗的递呈载体呈现非常好的应用前景,文章介绍了近年来乳酸乳球菌在疫苗递呈载体中的应用情况,重点阐述了其在抗感染、抗肿瘤、抗自身免疫性方面的研究进展及其在疫苗研制中遇到的困难及需要解决的问题,并对其应用前景进行了展望。

引用

页码：75 / 80

页数：6

共 21 条

[1] Expression of a hepatitis A virus antigen in Lactococcus lactis and Escherichia coli and evaluation of its immunogenicity [J].

Berlec, Ales ;

Malovrh, Tadej ;

Zadravec, Petra ;

Steyer, Andrej ;

Ravnikar, Matjaz ;

Sabotic, Jerica ;

Poljsak-Prijatelj, Mateja ;

Strukelj, Borut .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2013, 97 (10) :4333-4342

[2] Protection against human papillomavirus type 16-induced tumors in mice using non-genetically modified lactic acid bacteria displaying E7 antigen at its surface [J].

Ribelles, Pedro ;

Benbouziane, Bouasria ;

Langella, Philippe ;

Suarez, Juan E. ;

Bermudez-Humaran, Luis G. ;

Riazi, Ali .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2013, 97 (03) :1231-1239

[3] Oral immunization using live Lactococcus lactis co-expressing LACK and IL-12 protects BALB/c mice against Leishmania major infection [J].

Hugentobler, Felix ;

Di Roberto, Raphael B. ;

Gillard, Joshua ;

Cousineau, Benoit .

VACCINE, 2012, 30 (39) :5726-5732

[4] Bacterium-like particles supplemented with inactivated influenza antigen induce cross-protective influenza-specific antibody responses through intranasal administration [J].

de Haan, Aalzen ;

Haijema, Bert Jan ;

Voorn, Petra ;

Meijerhof, Tjarko ;

van Roosmalen, Maarten L. ;

Leenhouts, Kees .

VACCINE, 2012, 30 (32) :4884-4891

[5] Oral vaccination with Lactococcus lactis expressing the Vibrio cholerae Wzm protein to enhance mucosal and systemic immunity [J].

Zamri, Hana Farizah ;

Shamsudin, Mariana Nor ;

Rahim, Raha Abdul ;

Neela, Vasanthakumari .

VACCINE, 2012, 30 (21) :3231-3238

[6]

Oral immunization of mice with recombinant Lactococcus lactis expressing Cu,Zn superoxide dismutase of Brucella abortus triggers protective immunity[J] . Vaccine . 2011 (7)

[7]

Hsp65-producing Lactococcus lactis prevents experimental autoimmune encephalomyelitis in mice by inducing CD4+LAP+ regulatory T cells[J] . Journal of Autoimmunity . 2012

[8]

Mucosal priming of the murine immune system against enterohemorrhagic Escherichia coli O157:H7 using Lactococcus lactis expressing the type III secretion system protein EspB[J] . B. Ahmed,M. Loos,D. Vanrompay,E. Cox.Veterinary Immunology and Immunopathology . 2012

[9] Reversal of autoimmune diabetes by restoration of antigen-specific tolerance using genetically modified Lactococcus lactis in mice [J].

Takiishi, Tatiana ;

Korf, Hannelie ;

Van Belle, Tom L. ;

Robert, Sofie ;

Grieco, Fabio A. ;

Caluwaerts, Silvia ;

Galleri, Letizia ;

Spagnuolo, Isabella ;

Steidler, Lothar ;

Van Huynegem, Karolien ;

Demetter, Pieter ;

Wasserfall, Clive ;

Atkinson, Mark A. ;

Dotta, Francesco ;

Rottiers, Pieter ;

Gysemans, Conny ;

Mathieu, Chantal .

JOURNAL OF CLINICAL INVESTIGATION, 2012, 122 (05) :1717-1725

[10] New and future immunomodulatory therapy in type 1 diabetes [J].

Tooley, James E. ;

Waldron-Lynch, Frank ;

Herold, Kevan C. .

TRENDS IN MOLECULAR MEDICINE, 2012, 18 (03) :173-181

← 1 2 3 →