非靶向代谢组学生物样品采集和制备方法探讨

被引：44

作者：

冯利

曹芳瑞

刘新民

潘瑞乐

廖永红

常琪

机构：

[1] 中国医学科学院北京协和医学院药用植物研究所

来源：

中南药学 | 2014年 / 12卷 / 12期

关键词：

非靶向代谢组学; 生物样品采集; 生物样品制备;

D O I：

暂无

中图分类号：

R943 [制剂学]; Q503 [生物化学技术];

学科分类号：

070307 [化学生物学]; 100702 [药剂学];

摘要：

生物样品采集和制备是代谢组学研究的第一步也是最关键的一步,合适的样品采集和制备方法是获得可靠结果的重要保证。样品采集和制备方法与分析通量、分析成本和基质效应等密切相关。样品采集与制备方法如不进行充分验证,实验数据常出现较大偏差,但在实际工作中样品采集和制备过程常被忽视。本文对非靶向代谢组学生物样品常用采集和制备方法作一综述,指明了实际操作中的注意事项,有助于我们根据实验目的和样品性质选择合适的方法。本文重点关注基于高效液相色谱-质谱(HPLC-MS)和气相色谱-质谱(GC-MS)的代谢组学研究中常用样品采集和制备方法。

引用

页码：1217 / 1221

页数：5

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