干红葡萄酒中顺、反白藜芦醇及其苷的HPLC分析

被引：5

作者：

吴波

张寒俊

机构：

[1] 武汉工业学院湖北省粮食加工与转化重点实验室

来源：

中国酿造 | 2006年 / 09期

关键词：

高效液相色谱; 葡萄酒; 异构体; 白藜芦醇苷; 白藜芦醇; 荧光检测器;

D O I：

暂无

中图分类号：

TS262.6 [葡萄酒、香槟酒];

学科分类号：

100403 [营养与食品卫生学];

摘要：

采用PolarisC18-A色谱柱(100×2.0mmid,3μm),PhenomenexC18预柱(4×3.0mmid),乙腈、水为流动相,梯度洗脱,流速0.4mL/min,柱温40℃,荧光检测器:激发波长334nm;发射波长404nm,直接进样,同时分离了顺、反白藜芦醇及其苷。结果显示:在选定色谱条件下,白藜芦醇及其苷的顺、反异构体线性关系良好,加样平均回收率为90.7%￣98.5%;相对标准偏差为0.51%￣1.68%。该方法精密度好,结果可靠,适合于红酒中顺、反白藜芦醇及其苷的定量分析。

引用

页码：64 / 67

页数：4

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