Yi-Qi-Jian-Pi formula ameliorates immune function in acute-on-chronic liver failure by upregulating autophagy and mitochondrial biogenesis in CD8+T lymphocytes

被引：15

作者：

Tang, Li ^{[1
,2
]}

Wang, Xi ^{[1
]}

Zhao, Rong ^{[1
]}

Chen, Xiaomei ^{[1
]}

Wang, Feixia ^{[3
]}

Xia, Siwei ^{[3
]}

Xiao, Qian ^{[1
]}

Zhao, Qiang ^{[1
]}

Yang, Shiyan ^{[1
]}

Tan, Shanzhong ^{[1
]}

机构：

[1] Nanjing Univ Chinese Med, Nanjing Hosp, Dept Integrated TCM & Western Med, Nanjing 210003, Peoples R China

[2] Nanjing Univ Chinese Med, Nanjing Hosp Chinese Med, Dept Gastroenterol, Nanjing 210022, Peoples R China

[3] Nanjing Univ Chinese Med, Sch Pharm, Jiangsu Key Lab Pharmacol & Safety Evaluat Chinese, Nanjing 210023, Peoples R China

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2023年 / 308卷

关键词：

Yi-Qi-Jian-Pi formula; Mitochondrial biogenesis; Autophagy; CD8+T lymphocytes; Acute -on -chronic liver failure; T-CELLS;

D O I：

10.1016/j.jep.2023.116276

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Ethnopharmacological relevance: A key event in the pathogenesis of acute-on-chronic liver failure (ACLF) is the imbalance in the systemic immune response; immunosuppression in patients with ACLF contributes to poor prognosis. The Yi-Qi-Jian-Pi formula (YQJPF) may improve T lymphocyte immune function in patients with ACLF.Aim of the study: To investigate the immune mechanism of YQJPF in vivo and in vitro.Materials and methods: An ACLF rat model was established by injection of CCl4, lipopolysaccharide, and D-galactosamine. We examined the effect of different doses of YQJPF (6.43, 12.87, 25.74 g/kg) on liver injury and immune function in the ACLF rat model. Magnetic-activated cell sorting was used to sort the CD8+ T lymphocytes in the spleen for lymphocyte function detection. In primary CD8+ T lymphocytes and Jurkat cell lines, the expression of mitochondrial function and biogenesis and autophagy related markers were detected using mo-lecular biological methods and flow cytometry analysis.Results: YQJPF improved the peripheral blood lymphocyte count and proportion of CD8+ T lymphocytes in ACLF rats, increased pro-inflammatory factors (IL-2, IFN-lambda, and TNF-alpha), and reduced anti-inflammatory factors (IL-10 and TGF beta 1). YQJPF also improved metabolism and mitochondrial homeostasis in CD8+ T lymphocytes, alle-viated lymphocyte immune dysfunction by promoting autophagy, upregulated mitochondrial biogenesis by promoting PGC-1 alpha, NRF-1, and TFAM expression, and regulated the relationship between autophagy and mitochondrial biogenesis via PGC-1 alpha. Conclusions: Our results suggest that YQJPF could improve immune function in a rat model of ACLF, possibly via affecting the homeostasis of lymphatic mitochondria in CD8+ T lymphocytes. YQJPF may enhance lymphocyte mitochondrial biosynthesis and promote lymphocyte autophagy. PGC-1 alpha is a possible upstream regulatory target of YQJPF.

引用

页数：13

共 40 条

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