Single-molecule DNA sequencing technologies for future genomics research

被引:133
作者
Gupta, Pushpendra K. [1 ]
机构
[1] Chaudhary Charan Singh Univ, Dept Genet & Plant Breeding, Mol Biol Lab, Meerut 250004, Uttar Pradesh, India
关键词
D O I
10.1016/j.tibtech.2008.07.003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
During the current genomics revolution, the genomes of a large number of living organisms have been fully sequenced. However, with the advent of new sequencing technologies, genomics research is now at the threshold of a second revolution. Several second-generation sequencing platforms became available in 2007, but a further revolution in DNA resequencing technologies is being witnessed in 2008, with the launch of the first single-molecule DNA sequencer (Helicos Biosciences), which has already been used to resequence the genome of the M13 virus. This review discusses several single-molecule sequencing technologies that are expected to become available during the next few years and explains how they might impact on genomics research.
引用
收藏
页码:602 / 611
页数:10
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